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Acute Toxicity and Cytotoxicity Effect of Ethanolic Extract of Spondias tuberosa Arruda Bark: Hematological, Biochemical and Histopathological Evaluation Anais da ABC (AABC)
BARBOSA,HUMBERTO M.; NASCIMENTO,JAILSON N. DO; ARAÚJO,THIAGO A.S.; DUARTE,FILIPE S.; ALBUQUERQUE,ULYSSES P.; VIEIRA,JEYMESSON R.C.; SANTANA,EDSON R.B. DE; YARA,RICARDO; LIMA,CLÁUDIA S.A.; GOMES,DAYANE A.; LIRA,EDUARDO C..
ABSTRACT Spondias tuberosa Arruda, popularly named as umbu, is native from savanna-like vegetation and widely used for medicinal purposes, however, the toxicological profile is not available yet. This study evaluated the phytochemical profile and acute toxicity and citoxicity of Ethanolic Extract of Spondias tuberosa Arruda Bark (EEStb) in hematological, biochemical and histopathological parameters. Female Wistar rats were divided into: control (C) and animal treated single doses of 300mg/Kg (EEStb300) or 2.000mg/kg body weight (ESStb2.000) of the EEStb. After 24 hours and 14 days from gavage, the behavior, hematological, biochemical and histopathological parameters were assayed. Cytotoxicity effect was evaluated on HEp-2 cell lines. Neither EEStb300 nor...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acute toxicity; Cytotoxicity; Phytomedicine; Safety; Spondias tuberosa Arruda; Toxicology.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000501993
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Acute toxicity of pyrazosulfuron-ethyl and permethrin to juvenile Litopenaeus vannamei - doi: 10.4025/actascibiolsci.v33i1.6974 Biological Sciences
Mello, Giovanni Lemos de; EPAGRI; Amaral Júnior, Hilton; Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina - Epagri; Garcia, Silvano; Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina - Epagri; Vinatea, Luis; UFSC.
The objective of this study was to determine the LC50 (96h) of two pesticides: Sirius® 250 SC herbicide of the pyrazosulfuron-ethyl group, and Talcord® insecticide of the permethrin group, on juvenile Litopenaeus vannamei. Shrimp total hemocyte count (THC) was also determined as an indication of physiological alterations caused by the pesticides. Juvenile shrimp (5.0 ± 0.5 g) were exposed to the following concentrations: 0, 0.1, 1.0, 10, 100 and 1000 µg L-1 Sirius® 250 SC; and 0, 0.001, 0.01, 0.1, 1.0 and 10 µg L-1 Talcord®. The Talcord® LC50 (96h) was of 0.00933 µg L-1 or 9.33 ng L-1. There were no significant changes in the THC between control and test groups. No...
Palavras-chave: Toxicology; Pesticides; Shrimp culture; Litopenaeus vannamei Toxicology; Pesticides; Shrimp culture; Litopenaeus vannamei.
Ano: 2011 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6974
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Appraisal of acute oral toxicity of glucuronoxylan hydrogel from Mimosa pudica seeds BJPS
Muhammad,Gulzar; Hussain*,Muhammad Ajaz; Ashraf,Muhammad Umer; Naeem-ul-Hassan,Muhammad; Bukhari,Syed Nasir Abbas.
Glucuronoxylan hydrogel (GXH) isolated from M. pudica seeds was assessed for acute toxicology in albino mice that were alienated into four groups. Three groups, i.e., II, III and IV received GXH at a dose of 1, 2 and 5 g/kg, respectively while group I was retained untreated and provided routine diet. After administering GXH, mice were examined for vomiting, diarrhea, allergy and tremors for 8 h. All animals were carefully observed for food and water consumption at 1, 2, 3, 7 and 14 day after administering GXH. At the end of studies, blood samples were drawn for investigation of hematological and biochemical parameters. All animals were sacrificed, relative body weight of vital organs was calculated and their histopathology was studied. It was concluded...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glucuronoxylan; Acute toxicity; Histopathology; Toxicology; Mimosa pudica.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300619
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Aspectos patológicos da intoxicação por aceturato de diminazeno em camelídeos sul-americanos. Repositório Alice
SEIXAS, J. N.; ORLANDO, D. R.; WOUTERS, F.; WOUTERS, A. T. B.; VARASCHIN. M. S.; Raymundo, D. L..
Aceturato de diminazeno é um fármaco quimioterápico sintético comumente usado na medicina veterinária para o tratamento de doenças causadas por parasitos hematozoários. Entretanto, seu uso pode levar a efeitos colaterais, como alterações neurológicas graves e morte. A criação de camelídeos é uma atividade recente no Brasil, fazendo-se necessário conhecer mais sobre as doenças que acometem essas espécies. De dez camelídeos (seis lhamas e quatro alpacas) da propriedade, seis tiveram sinais clínicos e, destes, apenas uma lhama com manifestações leves recuperou-se. Os sinais clínicos incluíam apatia, andar cambaleante, fraqueza, sialorreia, cabeça baixa e pendida lateralmente, dificuldade em levantar e dispneia, observados a partir de 18 horas após o uso do...
Tipo: Artigo de periódico Palavras-chave: Diminazene aceturate; Neuropatologia; Terapia antiprotozoário; Llama.; Alpaca; Toxicologia; Neuropathology; Toxicology; Diminazene..
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1089291
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Biodegradation and toxicological evaluation of lubricant oils BABT
Tamada,Ivo Shodji; Lopes,Paulo Renato Matos; Montagnolli,Renato Nallin; Bidoia,Ederio Dino.
The aim of this work was to compare different toxicity levels of lubricant oils. The tests were performed using the earthworm (Eisenia andrei), arugula seeds (Eruca sativa) and lettuce seeds (Lactuca sativa), with three types of contaminants (mineral lubricant oil, synthetic lubricant oil and used lubricant oil) for various biodegradation periods in the soil. The toxicity tests indirectly measured the biodegradation of the contaminants. The samples were analyzed at t0, t60, t120 and t180 days of biodegradation. The used lubricant oil was proved very toxic in all the tests and even after biodegradation its toxicity was high. The mineral and synthetic oils were biodegraded efficiently in the soil although their toxicity did not disappear completely after 180...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Toxicology; Biodegradation; Lubricant oil; Toxicity bioassay.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000600020
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Bothrops alternatus snake venom induces apoptosis of skeletal muscle cells in a rabbit model Arq. Bras. Med. Vet. Zootec.
Santos,W.G.; Silveira,T.L.; Fiúza,A.T.L.; Botelho,A.F.M.; Gonçalves,I.N.N.; Ferreira,E.; Soto-Blanco,B.; Melo,M.M..
RESUMO Este estudo objetiva reportar a ocorrência de apoptose in vivo induzida pelo veneno da serpente Bothrops alternatus em células musculares esqueléticas. Cinco coelhos machos, adultos, receberam 150μg/kg de veneno no músculo vasto lateral, enquanto outros cinco animais receberam 0,1% de BSA diluído em PBS no mesmo local. Após 12 horas, os animais foram eutanasiados, e amostras do local de inoculação foram coletadas para análise histopatológica. Foram evidenciadas necrose e hemorragia nas células musculares. Além disso, a análise imuno-histoquímica para identificação de caspase-3 ativada revelou marcações granulares e agregadas no citoplasma das células musculares, compatíveis com o processo de apoptose. Este é o primeiro relato que confirma o veneno...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Snake venom; Botropic venom; Apoptosis; Toxicology; Rabbit.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000100349
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Capacidade de paras¡tismo de Telenomus podisi em ovos de Euschistus heros tratados com ¡nseticidas. Repositório Alice
CASTELLANOS, N. L.; HADDI, K.; BUENO, A. de F.; HIROSE, E.; SMAGGHE, G.; OLIVEIRA, E. E..
Conteúdo do volume 2: Ácaros; Biologia, fisiologia, morfologia; Controle biológico com bactérias entomopatogênicas; Controle biológico com fungos entomopatológicos; Controle biológico com nematoides; Controle biológico com parasitoides; Controle biológico com predadores; Ecologia e biodiversidade; Educação e etnoentomologia; Entomologia florestal; Entomologia Forense; Entomologia médica e veterinária; Entomologia molecular; Manejo integrado de pragas; Organismos geneticamente modificados; Plantas inseticidas; Polinização; Pragas quarentenárias e invasivas; Resistência de insetos a táticas de controle; Resistência de plantas a insetos; Semioquímicos e comportamento; Sistemática e taxonomia; Tecnologia de aplicação; Controle biológico com vírus...
Tipo: Separatas Palavras-chave: Parasitismo; Toxicologia; Controle Biológico; Soja; Soybeans; Toxicology; Biological control; Parasitism.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1099950
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DNA damage and cytotoxicity in pathology laboratory technicians exposed to organic solvents Anais da ABC (AABC)
AQUINO,TATIANE DE; ZENKNER,FERNANDA F.; ELLWANGER,JOEL H.; PRÁ,DANIEL; RIEGER,ALEXANDRE.
The aim of this study was to evaluate potential DNA damage and cytotoxicity in pathology laboratory technicians exposed to organic solvents, mainly xylene. Peripheral blood and buccal cells samples were collected from 18 technicians occupationally exposed to organic solvents and 11 non-exposed individuals. The technicians were sampled at two moments: Monday and Friday. DNA damage and cytotoxicity were evaluated using the Comet Assay and the Buccal Micronucleus Cytome assay. Fifteen subjects (83.5%) of the exposed group to solvents complained about some symptom probably related to contact with vapours of organic solvents. DNA damage in the exposed group to solvents was nearly 2-fold higher on Friday than on Monday, and in both moments the individuals of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA damage; Micronucleus; Toxicology; Occupational health; Organic solvents; Xylene.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000100227
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Effect of Lamivudine on the Rat Pregnancy Outcome International Journal of Morphology
Pontes,Rosana Dorsa Vieira; Amed,Abês Mamed; Simões,Manuel Jesus; Oliveira-Filho,Ricardo Martins; Simões,Ricardo Santos; Kulay Jr,Luiz.
Human immunodeficiency virus (HIV) infection is in growing incidence throughout the world. Due to the increasing proportion of affected women in reproductive years, the association of pregnancy with HIV infection becomes a matter of major Public Health concern. Antiretroviral drug administration turned out to be imperative during pregnancy in order to prevent the vertical transmission; accordingly, new antiretroviral drugs and anti-HIV drug associations have been tested in experimental pregnancy models before they are approved to be included in protocols for use during human pregnancy. Lamivudine is a nucleoside reverse transcriptase inhibitor currently used in association with other antiretrovirals. Since no data exist on the perinatal safety of...
Tipo: Journal article Palavras-chave: Lamivudine; Toxicology; Rat; Pregnancy.
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022005000300001
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EFFECTS OF PYRAZINAMIDE ON PREGNANT ALBINO RATS International Journal of Morphology
Pascale Quintino,Marisa; Simões,Manuel de Jesus; Uchiyama Nakamura,Mary; Martins Oliveira-Filho,Ricardo; Espiridião,Silvia; Kulay Júnior,Luiz.
Although being used for years in the treatment of tuberculosis, no data are available in the literature on the safety of pyrazinamide during pregnancy. Accordingly, we aimed to make a first approach to this problem by evaluating the effects of this drug administered during the entire pregnancy of albino rats. Fourty female, EPM-1 Wistar albino rats of about 250 g b.w. were used. Upon conception (day zero of pregnancy) the animals were randomly divided in 4 groups of 10 rats each and labeled as follows. Controls (C), animals treated with the drug vehicle (destilled water); experimental groups (E1, E2 and E3), animals treated with 35, 105 or 315 mg/kg b.w. pyrazinamide by gavage (oral route) once daily up to the term (20th day of pregnancy). Drug or vehicle...
Tipo: Journal article Palavras-chave: Pyrazinamide; Toxicology; Rat; Pregnancy.
Ano: 2003 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022003000400007
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Effects of wastewater from oil exploration on soil mesofauna. Repositório Alice
FERREIRA, R. N. C.; WEBER, O. B.; CORREIA, M. E. F.; IKEDA, E. S. B.; SCORIZA, R. N.; MESQUITA, A. L. M..
Tipo: Artigo de periódico Palavras-chave: Glutaraldehyde; Soil fauna; Toxicology; Water reuse..
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1032316
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Evaluation of the Toxicity of Silver/Silica and Titanium Dioxide Particles in Mammalian Cells BABT
Pittol,Michele; Tomacheski,Daiane; Simões,Douglas Naue; Ribeiro,Vanda Ferreira; Santana,Ruth Marlene Campomanes.
ABSTRACT The increasing concern over the spread of diseases has lead to a high consumption of antimicrobial additives in the medical and industrial fields. Since these particles can lixiviate from loaded materials, the contact between this additive and mammalian cells can occur during manufacture, use and disposal of the products. Silver on fumed silica (AgNP_SiO2) and titanium dioxide (TiO2) can be used as antimicrobial additives that are applied in polymeric formulation. While these additives can inhibit bacteria, fungus and virus proliferation; they may also be harmful to humans. Standard toxicological studies were undertaken using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide), CBPI (cytokinesis-block proliferation index) and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Titanium dioxide; Silver; Silica; Toxicology; Mammalian cell.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100318
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Histological Evaluation of the Effects of Borax Obtained from Various Sources in Different Rat Organs International Journal of Morphology
Kabu,Mustafa; Tosun,Murat; Elitok,Bülent; Akosman,Murat Sirri.
Boron is an essential element for life and intake via different sources into the body. Because effects of boron and compounds on the body has not been studied enough especially in tissue level, we planned this study to evaluate the effects of borax the most intaken form of boron compound on different intraabdominal organs histologically and also clinically. 42 male rats divided into equal 7 groups and different toxicological doses consistent with its LD50 dose (5000 mg/kg/d) were administered by gavage except control and sham groups. In the study, 2 different kinds of borax one of which was produced for research and the other for agriculture but the same formulation, were used and their effects were also compared. As a result it was found that borax did...
Tipo: Journal article Palavras-chave: Boron; Borax; Inflammatory bowel disease; Histopathology; Toxicology.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000100040
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Impact of atrazine on somatic aneuploidy in cupped oysters, Crassostrea gigas ArchiMer
Bouilly, Karine; Leitao, Alexandra; Mccombie, Helen; Chaves, R.; Guedes Pinto, H; Boudry, Pierre; Lapegue, Sylvie.
In the Pacific oyster, Crassostrea gigas hypodiploid aneuploid cells with 2n=19, 18 or 17 chromosomes have regularly been reported. There is a negative correlation between somatic aneuploidy and growth rate in oysters (Leitão et al., 2001a) as well as evidence for a genetic basis (Leitão et al., 2001b). Furthermore, non-random chromosome loss has been demonstrated in aneuploid karyotypes of C. gigas using the G-banding technique (Leitão et al., 2001c). This poster summarises different studies used to investigate the effects of the herbicide atrazine on the level of aneuploidy in this species
Tipo: Text Palavras-chave: Genetic; Aneuploidy; Toxicology; Impact; Atrazine; Crassostrea gigas; Pacific oysters.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/acte-3489.pdf
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Manejo de agrotóxico e qualidade ambiental:manual técnico. Infoteca-e
CHAIM, A.; FRIGHETTO, R. T. S.; VALARINI, P. J..
Este manual traz informações sobre cuidados no manuseio e uso de agrotóxicos, apresentando técnicas adequadas de aplicação, com vistas à redução de perdas e minimização da contaminação ambiental, humana e animal. Foi exclusivamente preparado para servir de guia nos treinamentos de técnicos em ciências agrárias, agricultores e aplicadores. Espera-se que os monitores possam multiplicar essas informações e, dessa forma, contribuir para a melhoria da qualidade ambiental.
Tipo: Folhetos Palavras-chave: Calibração; Tríplice lavagem; Meio ambiental; Agrotóxico; Pulverização; Método de aplicação; Toxicologia; Descarte; Pesticides; Pesticide application; Toxicology; Environment.
Ano: 1999 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/964433
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Masquerade, mimicry and crypsis of the polymorphic sea anemone Phyllodiscus semoni and its aggregations in South Sulawesi Naturalis
Hoeksema, B.; Crowther, A.L..
Phyllodiscus semoni is a morphologically variable sea anemone species from the Indo-Pacific with morphotypes ranging from upright and branched to low-lying and rounded. The apparent camouflage strategies of this sea anemone allow it to resemble other species or objects in its environment, such as stony corals, soft corals, seaweeds, or rocky boulders covered by algae, which may help it to avoid recognition by potential predators. Occasionally, it occurs in aggregations that may result from asexual reproduction. A high level of intraspecific morphological variation, including co-occurring aggregations of three different morphotypes, was observed in the Spermonde Archipelago off Makassar, South Sulawesi, Indonesia. The co-occurrence of aggregations with...
Tipo: Article / Letter to the editor Palavras-chave: Actiniaria; Aliciidae; Camouflage; Indonesia; Morphotype; Southern Japan; Toxicology; 42.72.
Ano: 2011 URL: http://www.repository.naturalis.nl/record/402023
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Meta-analysis of individual and combined effects of mycotoxins on growing pigs Scientia Agricola
Andretta,Ines; Kipper,Marcos; Hauschild,Luciano; Lehnen,Cheila Roberta; Remus,Aline; Melchior,Raquel.
ABSTRACT Little is known about the toxicity of concomitantly occurring mycotoxins in pig diets. This study was conducted to evaluate, through meta-analysis, the individual and the combined effects of mycotoxins on pig performance. The meta-analysis followed three sequential analyses (graphical, correlation, and variance-covariance) based on a database composed of 85 published papers, 1,012 treatments and 13,196 animals. Contamination of diets with individual mycotoxins reduced (p < 0.05) feed intake by 14 % and weight gain by 17 %, while combined mycotoxins reduced the same responses by 42 % and 45 %, respectively, in comparison with the non-challenged group. The correlation (p < 0.05) between reduction in weight gain (ΔG) and reduction in feed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Modelling; Sanitary challenge; Swine; Toxicology.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162016000400328
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Modelo linear misto em estudos toxicológicos. Repositório Alice
OLIVEIRA, L. P. de; LIMA, C. G. de; MAIA, A. de H. N.; CASTRO, V. L. S. S. de.
O objetivo deste trabalho é buscar um modelo linear misto que melhor descreva a variabilidade dos pesos de filhotes de ratas, em um experimento com 3 níveis de agrupamentos hierárquicos: à rata (nível 1) estão aninhados os filhotes (nível 2) com observações repetidas ao longo de 23 dias (nível 3). O experimento tem como objetivo avaliar os efeitos do fungicida no crescimento de filhotes de ratas tratadas com dose 0, 25 ou 50mg/kg em uma das fases da gestação (inicial ou organogênese). O modelo linear misto possibilitou modelar: a variabilidade entre ratas, a variabilidade entre filhotes, a correlação entre filhotes de mesma rata, a estrutura de correlação dos erros associados às pesagens diárias no filhote e entre as pesagens diárias de filhotes da mesma...
Tipo: Anais e Proceedings de eventos Palavras-chave: Fungicida; Rato; Toxicologia; Modelo matemático; Análise estatística; Rats; Fungicides; Toxicology; Linear models.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/968653
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Morphometric Alterations Induced by the Toxicity of Variable Sizes of Silver Nanoparticles International Journal of Morphology
Almansour,Mansour; Jarrar,Qais; Battah,Abdelkader; Jarrar,Bashir.
Silver nanoprticles (SNPs) are invested in medical, industrial and environmental applications. Little if any, is known about the morphometric alterations induced by the toxicity of SNPs. The aim of the present work is to find out the effect of variable size of SNPs on different morphometric parameters. Adult healthy male mice (BAL/C) were subjected to (10 nm, 20 nm, 40 nm 60 nm and 100 nm) SNPs for 35 days. Silver NPs caused non-significant decline on the average weight, significant decline in food consumption, increase in water intake, unilateral blindness, tanning fur color and cholestasis together with a decrease in the relative ratio of the liver, kidney and spleen weight to body weight. Mice subjected to 10 nm and 20 nm were more affected than mice...
Tipo: Journal article Palavras-chave: Silver nanoparticles; Morphology; Toxicology; Liver; Kidney; Spleen.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000200022
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Non-clinical studies in the process of new drug development - Part II: Good laboratory practice, metabolism, pharmacokinetics, safety and dose translation to clinical studies BJMBR
Andrade,E.L.; Bento,A.F.; Cavalli,J.; Oliveira,S.K.; Schwanke,R.C.; Siqueira,J.M.; Freitas,C.S.; Marcon,R.; Calixto,J.B..
The process of drug development involves non-clinical and clinical studies. Non-clinical studies are conducted using different protocols including animal studies, which mostly follow the Good Laboratory Practice (GLP) regulations. During the early pre-clinical development process, also known as Go/No-Go decision, a drug candidate needs to pass through several steps, such as determination of drug availability (studies on pharmacokinetics), absorption, distribution, metabolism and elimination (ADME) and preliminary studies that aim to investigate the candidate safety including genotoxicity, mutagenicity, safety pharmacology and general toxicology. These preliminary studies generally do not need to comply with GLP regulations. These studies aim at...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Non-clinical studies; GLP studies; Safety; Pharmacokinetics; Toxicology.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001200301
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