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Transgene inheritances and genetic similarities of near isogenic lines of genetically modified common beans PAB
Pinheiro,Patrícia Valle; Faria,Josias Corrêa de; Nogueira,Elsa Oliveira Paranaguá e Lago; Aragão,Francisco José Lima.
The objective of the present work was to determine the inheritance and stability of transgenes of a transgenic bean line expressing the genes rep-trap-ren from Bean golden mosaic virus and the bar gene. Crosses were done between the transgenic line and four commercial bean cultivars, followed by four backcrosses to the commercial cultivars. Progenies from each cross were evaluated for the presence of the transgenes by brushing the leaves with glufosinate ammonium and by polymerase chain reaction using specific oligonucleotides. Advanced generations were rub-inoculated with an isolate of Bean common mosaic necrosis virus (BCMNV). The transgenes were inherited consistently in a Mendelian pattern in the four crosses studied. The analyzed lines recovered close...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phaseolus; BCMNV; Genetic engineering; Genetic variability; Transgene genotypic stability.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009000900015
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