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Registros recuperados: 32
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A comparative expression analysis of gene transcripts in brain tissue of non-transgenic and GH-transgenic zebrafish (Danio rerio) using a DDRT-PCR approach Anais da ABC (AABC)
Alves-Costa,Fernanda A.; Figueiredo,Márcio A.; Lanes,Carlos F.C.; Almeida,Daniela V.; Marins,Luis F.; Wasko,Adriane P..
The presence of higher level of exogenous growth hormone (GH) in transgenic animals could lead to several physiological alterations. A GH transgenic zebrafish (Danio rerio) line was compared to nontransgenic (NT) samples of the species through a DDRT-PCR approach, with the goal of identifying candidate differentially expressed transcripts in brain tissues that could be involved in GH overexpression. Densitometric analyses of two selected amplification products, p300 and ADCY2, pointed to a significant lower gene expression in the transgenic zebrafish (104.02 ± 57.71; 224.10 ± 91.73) when compared to NT samples (249.75 ± 30.08; 342.95 ± 65.19). The present data indicate that p300 and ADCY2 are involved in a regulation system for GH when high circulating...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Differential display; GH; Growth hormone; Transgenic; Zebrafish.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000200017
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A practical approach to the understanding and teaching of RNA silencing in plants Electron. J. Biotechnol.
Bazzini,Ariel A; Mongelli,Vanesa C; Hopp,H. Esteban; del Vas,Mariana; Asurmendi,Sebastián.
Gene silencing, also called RNA interference (RNAi) is a specific mechanism of RNA degradation involved in gene regulation, development and defense in eukaryotic organisms. It became an important subject in the teaching programs of molecular biology, genetics and biotechnology courses in the last years. The aim of this work is to provide simple and inexpensive assays to understand and teach gene silencing using plants as model systems. The use of transient and permanent transgenic plants for expressing reporter genes, like those derived from jellyfish green fluorescent protein (gfp) encoding gene, provides a nice, colorful and conclusive image of gene silencing. Three experimental approaches to evidence RNA silencing are depicted. In the first approach...
Tipo: Journal article Palavras-chave: Gene silencing; GFP; HC-Pro; PDS; Protocols; PTGS; Teaching; Transgenic; Transient expression; TRV; VIGS.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200002
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Advances in Apple Transformation Technology to Confer Resistance to Fungal Diseases in Apple Crops: A Chilean Perspective Chilean J. Agric. Res.
Polanco,Víctor; Paredes,Mario; Becerra,Viviana; Pérez,Eduardo.
Apple (Malus domestica Borkh.) is one of the most consumed fruit in the world. Genetic transformation is a key process to sustain this demand by permitting the potential enhancement of existing cultivars as well as the development of new cultivars resistant to pests, diseases, and storage problems that occur in the major production areas. This review summarizes the advances of genetic engineering applied to the development of resistant apple cultivars to fungus disease, with particular attention in the generation of apples resistant to Venturia inaequalis (Cooke) G.Winter, the main phytosanitary problem that affects apple crops in Chile.
Tipo: Journal article Palavras-chave: Apple; Fungal disease; Transgenic; Cisgenesis.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392010000200013
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An improved method for transformation of lettuce by Agrobacterium tumefaciens with a gene that confers freezing resistance BABT
Pileggi,Marcos; Pereira,Albanin Aparecida Mielniczki; Silva,Joandrei dos Santos; Pileggi,Sônia Alvim Veiga; Verma,Desh Pal S..
An efficient method for constructing transgenic lettuce cultivars by Agrobacterium tumefaciens was described by Torres et al., 1993. In the present work, an improvement of the above procedure is described and applied to transform the cultivar Grand Rapids with a mutated P5CS gene. The major modifications were concerned with turning more practical the transformation and regeneration protocols. Also we tried to improve transformation steps by increasing injured area in explants and prolonging co-cultivation with Agrobacteria (in larger concentration). A more significant selective pressure was used against non-transformed plants and bacteria. In these work we were concerned to obtain T1 and T2 seeds. The P5CS gene codes for a delta¹-pyrroline-5-carboxylate...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Stress resistance; Cold resistance; Transgenic; Lettuce; Proline.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000200013
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Attitudes towards genomic: Research in four Latin American countries Electron. J. Biotechnol.
Rodríguez Yunta,Eduardo; Valdebenito Herrera,Carolina; Misseroni,Adelio; Fernández Milla,Lautaro; Outomuro,Delia; Schiattino Lemus,Irene; Ferrer Lues,Marcela; Lolas Stepke,Fernando.
The present reflection refers to data obtained about the social representations of genomic research and its applications through interviews with legislators and lawyers, biomedical researchers and civilians and the review of scientific and legal literature in four Latin American countries: Argentine, Chile, Mexico and Peru. Several issues are addressed: little access to prevention and therapeutic methods, lack of equity in health benefits, commercialization of gene sequences through patents which leads to commercial exploitation of underdeveloped countries, the possibility of physical or psychological damage or genetic discrimination, the possibility of genetic modifications or abortion for eugenic reasons, the necessity of safeguarding confidentiality,...
Tipo: Journal article Palavras-chave: Cloning; Genome; Social representations; Transgenic.
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000300003
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Biotechnology and Planted Forests: Assessment of Potential and Possibilities AgEcon
Sedjo, Roger A..
This paper addresses the potential impact of the introduction and development of biotechnology on planted forests. It includes a description of some recent innovations in forestry including the use of traditional breeding, and also more recent innovations involving biotechnology, including the development of clonal propagation and the use of modern molecular biology techniques. In addition to describing these innovations, the paper undertakes an assessment of their probable impact on future production of the forest industry, on the global timber supply, and on future markets for timber and wood products. The paper offers a description of recent innovations in tree breeding and biotechnology, including a discussion of innovations in agriculture that have...
Tipo: Working or Discussion Paper Palavras-chave: Biotechnology; Plantation forests; Genetic modification; Genetic research; Economic benefits; Transgenic; GMO; Resource /Energy Economics and Policy; Q21; Q23; Q16; O32; L73.
Ano: 1999 URL: http://purl.umn.edu/10862
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Caracterização genético-bioquímica de milho tropical transformado com o gene que codifica para a zeína. Repositório Alice
CARNEIRO, N. P.; CARNEIRO, A. A.; CARVALHO, C.H.S.; LOPES, M.A.; PAIVA, E..
2000
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Transgenico; Milho tropical; Zeinas; Metionina; Endosperma; Zea mays; Tropical maize; Transgenic; Zein; Endosperm.
Ano: 2000 URL: http://www.alice.cnptia.embrapa.br/handle/doc/484445
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Chemical control of stalk regrowth in glyphosate-resistant transgenic cotton AGRIAMBI
Ferreira,Alexandre C. de B.; Bogiani,Julio C.; Sofiatti,Valdinei; Silva Filho,João L. da.
ABSTRACT Cotton stalk regrowth must be controlled after harvest to ensure the phytosanitary quality and sustainability of cotton farming systems. Mechanical destruction generally does not conform to the no-tillage system, and the control of cotton stalk regrowth by herbicides is little studied in Brazil. The objective of this study was to evaluate the efficiency of herbicides to control glyphosate-resistant transgenic cotton stalk regrowth. The study was conducted in three environments of the Brazilian Cerrado region, two in Santa Helena de Goiás (SHGO), during 2013 and 2014, and one in Luís Eduardo Magalhães (LEM-BA) in 2014. In each of the three environments, the experiment was carried out in a randomized complete block with four replications. Each...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gossypium hirsutum; Transgenic; Stalk regrowth; Herbicides; Environment.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662018000800530
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Chitinase activities, scab resistance, mycorrhization rates and biomass of own-rooted and grafted transgenic apple Genet. Mol. Biol.
Schäfer,Tina; Hanke,Magda-Viola; Flachowsky,Henryk; König,Stephan; Peil,Andreas; Kaldorf,Michael; Polle,Andrea; Buscot,François.
This study investigated the impact of constitutively expressed Trichoderma atroviride genes encoding exochitinase nag70 or endochitinase ech42 in transgenic lines of the apple cultivar Pinova on the symbiosis with arbuscular mycorrhizal fungi (AMF). We compared the exo- and endochitinase activities of leaves and roots from non-transgenic Pinova and the transgenic lines T386 and T389. Local and systemic effects were examined using own-rooted trees and trees grafted onto rootstock M9. Scab susceptibility was also assessed in own-rooted and grafted trees. AMF root colonization was assessed microscopically in the roots of apple trees cultivated in pots with artificial substrate and inoculated with the AMF Glomus intraradices and Glomus mosseae. Own-rooted...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apple; Chitinase; Mycorrhiza; Transgenic.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300014
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Consumers Willingness to Pay to Avoid Transgenic Products AgEcon
Ribeiro, Francis Lee; de Noronha, Jose Ferreira; Noronha, Cassia Maria Silva.
The debate on transgenic, which is embebed in asymmetrical information, has as a focal point, the risks and uncertainties to them associated. Many are the concerns and questions about the risks for the human health and the environment. Considering the interests of the productive sector in the transgenic technology, the matter sets itself in terms of how fast it wants to be settled taking into consideration the incapability of establishing certainties about the damages or benefits within the process. With the recent liberation by the Brazilian government for its production the sector starts showing segmentation. Therefore, this issue reflects in the market itself. The question is: are the consumers willing to buy transgenic? As an attempt to answer this...
Tipo: Conference Paper or Presentation Palavras-chave: Transgenic; Contingent Valuation; Measure of Well-being; Demand and Price Analysis; Food Consumption/Nutrition/Food Safety.
Ano: 2005 URL: http://purl.umn.edu/24221
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Detection of adventitious presence of genetically modified seeds in lots of non transgenic soybean Rev. bras. sementes
Pádua,Gilda Pizzolante de; Jesus,Adriana Madeira Santos; Fronza,Vanoli; Arantes,Neylson Eustáquio; Zito,Roberto Kazuhiko.
The difficulty on identifying, lack of segregation systems and absence of suitable standards for coexistence of non trangenic and transgenic soybean are contributing for contaminations that occur during productive system. The objective of this study was to evaluate the efficiency of two methods for detecting mixtures of seeds genetically modified (GM) into samples of non-GM soybean, in a way that seed lots can be assessed within the standards established by seed legislation. Two sizes of soybean samples (200 and 400 seeds), cv. BRSMG 810C (non-GM) and BRSMG 850GRR (GM), were assessed with four contamination levels (addition of GM seeds, for obtaining 0.0%, 0.5%, 1.0%, and 1.5% contamination), and two detection methods: immunoassay of lateral flux (ILF) and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max; Glyphosate; Transgenic.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222012000400007
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Effects of high proline accumulation on chloroplast and mitochondrial ultrastructure and on osmotic adjustment in tobacco plants Agronomy
Borgo, Lucélia; Marur, Celso Jamil; Vieira, Luiz Gonzaga Esteves.
Proline accumulates in many organisms in response to abiotic stresses, such as drought and salinity. Reports have discussed the role of proline in relation to osmotic adjustment, even though no clear-cut evidence has yet been provided demonstrating this association as a defense mechanism against drought stress in plants. However, it has been indicated that the application of exogenous proline can damage the ultrastructure of chloroplasts and mitochondria. In this study, using transgenic tobacco plants carrying a mutant p5cs gene from Vigna aconitifolia under control of the CaMV 35S promoter, we show that the high levels of endogenous proline accumulated in leaves does not contribute to the osmotic adjustment in plants under water deficit conditions....
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.01.00.00-9 p5cs; Transgenic; Water deficit Melhoramento Vegetal.
Ano: 2015 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19097
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EL PAPEL DEL ADMINISTRADOR DE AGRONEGOCIOS EN LA SEGURIDAD ALIMENTARIA AgEcon
Guerra E., Guillermo A.; Aguilar Valdes, Alfredo; Cabral Martell, Agustin; Alvarado Martinez, Luis Felipe; Alvarado Martinez, Tomas Everardo; Arras Vota, Ana Maria; Moreno Medina, Salomon.
For decades, food security has been a worldwide problem affecting the poorest in order to find a possible solution to this dilemma that not only continues, but constantly grows with the increase in population. Agriculture, as the main food supplier, has evolved and increased its productivity, and has expanded its agricultural frontiers, since previous decades, in its attempts to solve this problem. If predominant conventional agriculture (mainly dedicated to productivity) is complemented with substantial increase of transgenic, organic, agroecological, greenhouse and hydroponic crops, it will be more feasible to avoid the loss of forest, the damage and loss of soils and at the same time diminish food insecurity. Within this context, the agribusiness...
Tipo: Journal Article Palavras-chave: Food security; Transgenic; Organics; Agro ecological; Hydroponics; Greenhouse; Agribusiness manager.; Agribusiness.
Ano: 2011 URL: http://purl.umn.edu/99477
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Engenharia genética: conceitos básicos, ferramentas e aplicações. Infoteca-e
CORDEIRO, M. C. R..
ABSTRACT: Basic concepts and methodologies concerning genetic engineering are presented in this review and is directed to people or professionales not familiarized to it. Moreover, it can be a document to clarity what is genetic engineering, the importance of its contributions to modern agriculture, medicine or livestock research and to support discussions on genetic research ethics. Covers information on structure of nucleic acids, types, functions and main processes such us DNA duplication, transcription and traduction. Important methodologies are covered about DNA/RNA extraction, electrophoresis, PCR, nucleic acid libraries production, cloning, sequencing, gene characterization in connection to main historical events of genetic engineering. It...
Tipo: Documentos (INFOTECA-E) Palavras-chave: Engenharia genética; Biotecnologia; Marcador genético; DNA; RNA; Ácido nucléico; Gene; Biologia molecular; Clonagem; Transgênico; Genetic engineering; Biotechnology; Genetic markers; Nucleic acids; Genes; Molecular biology; Cloning; Transgenic.
Ano: 2003 URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/568132
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Ensaio de competição de cultivares tolerantes ao glifosato da rede soja sul de pesquisa, safra 2009/10. Infoteca-e
BERTAGNOLLI, P. F.; COSTAMILAN, L. M.; NICOLAU, M.; VERNETTI JUNIOR, F. de J.; STECKLING, C.; ROVERSI, T.; RUBIN, S. de A. L.; GONÇALVES, J. A.; CASTRO, R. L. de; GABE, N. L.; OLIVEIRA, M. A. R. de; VICENTE, D.; MATZENBACHER, R.; GIASSON, N. F.; BAGATINI, N. P.; SILVA, A. E. L. da..
2010
Tipo: Capítulo em livro técnico (INFOTECA-E) Palavras-chave: Soybean; Transgenic.
Ano: 2010 URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/882340
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Ex-Ante Evaluation of Drought Tolerant Varieties in Eastern and Central Africa AgEcon
Kostandini, Genti; Mills, Bradford F.; Mykerezi, Elton.
Tipo: Conference Paper or Presentation Palavras-chave: Transgenic; Drought Tolerance; Household Benefits; Consumer/Household Economics; Crop Production/Industries.
Ano: 2008 URL: http://purl.umn.edu/49372
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Genetic approaches for studying transgene inheritance and genetic recombination in three successive generations of transformed tobacco Genet. Mol. Biol.
Tizaoui,Kalthoum; Kchouk,Mohamed Elyes.
Transgene integration into plant genomes is a complex process accompanied by molecular rearrangements. Classic methods that are normally used to study transgenic population genetics are generally inadequate for assessing such integration. Two major characteristics of transgenic populations are that a transgenic genome may harbor many copies of the transgene and that molecular rearrangements can create an unstable transgenic locus. In this work, we examined the segregation of T1, T2 and T3 transgenic tobacco progenies. Since transfer DNA (T-DNA) contains the NptII selectable marker gene that confers resistance to kanamycin, we used this characteristic in developing a method to estimate the number of functional inserts integrated into the genome. This...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Copy number variation; Gene rearrangements; Genetic inheritance; Transgenic.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400015
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Genetic transformation of coffee Braz. J. Plant Physiol.
Ribas,Alessandra Ferreira; Pereira,Luiz Filipe Protasio; Vieira,Luiz Gonzaga E..
In the last 15 years, considerable advances were made in coffee genetic transformation. Different research groups in the world have been able to transform coffee with genes for insect resistance, decaffeinated coffee, herbicide resistance and control of fruit maturation. Although the majority of the research is still limited to laboratory and greenhouse studies, initial field tests with transformed coffee are beginning to appear in the literature. In this review we provide an update on the state of coffee genetic transformation, presenting technical aspects related to tissue culture systems, strategies for selection and transformation with particle bombardment, as well as the use of Agrobacterium tumefaciens. We also discuss the potential applications of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agrobacterium; Coffea; Biolistic; Genetic engineering; Transgenic.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000100007
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Genomics and genetic transformation in arachis. Repositório Alice
BRASILEIRO, A. C. M.; ARAUJO, A. C. G.; LEAL BERTIOLI, S. C.; GUIMARAES, P. M..
2014
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Arachis; Functional genomics; Transgenic; Genome; Peanut.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1006434
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GMP-RAM V1.1: uma proposta de software como sistema de suporte à decisão para avaliação de risco de plantas geneticamente modificadas. Repositório Alice
JESUS, K. R. E. de; VIEIRA, F. D.; LIMA, D. U. de.
ABSTRACT: An essential step in the development of products based upon genetically modified plants (GMP) is an assessment of safety, including an evaluation of potential impact on the crops and the practices related to their cultivation on the environment, human or animal health, in a comparative way with its parental or reference crop. Despite the importance of the evaluation being currently done by risk assessment, the ?GMP-RAM? is the first electronic tool for decision support at the evaluation of potential risks related to the use of genetically modified plants. The Software GMP-RAM is provided as a tool that may help to evaluate the safety of the genetically modified plants. The use of this software could represent a less subjective and more...
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Transgênicos; Avaliação de risco; Suporte à decisão; Transgenic; Biossegurança; Biosafety; Genetically modified organisms; Decision support systems; Risk assessment.
Ano: 2007 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/4710
Registros recuperados: 32
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