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| Hirumi, Hiroyuki; Hirumi, K.; Moloo, S. K.; Shaw, M. K.. |
| Bloodstream trypomastigotes (BSFs) of 2 clones (IL 1392 and IL 3671) of Trypanosoma vivax were cultured without feeder layers in 3 systems. System I: metacyclic-producing cultures of T. vivax IL 1392 were initiated with BSFs derived from infected mice at 27 OC in the presence of feeder layers using TVM-1 medium. Metacyclics harvested were then transferred to flasks containing feeder cells and maintained at 34 OC using TVM-22 medium. Under such conditions, the metacyclics transformed to BSFs. Bloodstream trypomastigotes which were then transferred to new flasks, continued to grow in HMI-162 medium without feeder cells. The maximum density of the BSFs and their shortest population doubling time were 3.5 x l06 / ml and 13.5 h, respectively. System II:... |
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Palavras-chave: Feeder cell layers; In vitro cultivation; Trypomastigotes. |
| Ano: 1991 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/153 |
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| Mortara,Renato A.; Andreoli,Walter K.; Taniwaki,Noemi N.; Fernandes,Adriana B.; Silva,Claudio V. da; Fernandes,Maria Cecília D.C.; L'abbate,Carolina; Silva,Solange da. |
| Trypanosoma cruzi, the etiological agent of Chagas disease, occurs as different strains or isolates that may be grouped in two major phylogenetic lineages: T. cruzi I, associated with the sylvatic cycle and T. cruzi II, linked to the human disease. In the mammalian host the parasite has to invade cells and many studies implicated the flagellated trypomastigotes in this process. Several parasite surface components and some of host cell receptors with which they interact have been identified. Our work focused on how amastigotes, usually found growing in the cytoplasm, can invade mammalian cells with infectivities comparable to that of trypomastigotes. We found differences in cellular responses induced by amastigotes and trypomastigotes regarding... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Trypanosoma cruzi; Cellular invasion; Amastigotes; Trypomastigotes; Parasitophorous vacuole escape; Trafficking; Coxiella burnetii; Phylogenetic lineages. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652005000100006 |
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| Yoshida,Nobuko. |
| Establishment of infection by Trypanosoma cruzi, the agent of Chagas' disease, depends on a series of events involving interactions of diverse parasite molecules with host components. Here we focus on the mechanisms of target cell invasion by metacyclic trypomastigotes (MT) and mammalian tissue culture trypomastigotes (TCT). During MT or TCT internalization, signal transduction pathways are activated both in the parasite and the target cell, leading to Ca2+ mobilization. For cell adhesion, MT engage surface glycoproteins, such as gp82 and gp35/50, which are Ca2+ signal-inducing molecules. In T. cruzi isolates that enter host cells in gp82-mediated manner, parasite protein tyrosine kinase as well as phospholipase C are activated, and Ca2+ is released from I... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Trypanosoma cruzi; Trypomastigotes; Cell invasion; Signal transduction; Ca2+ mobilization. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652006000100010 |
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