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Registros recuperados: 15
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Characterization of abalone Haliotis tuberculata-Vibrio harveyi interactions in gill primary cultures ArchiMer
Pichon, Delphine; Cudennec, Benoit; Huchette, Sylvain; Djediat, Chakib; Renault, Tristan; Paillard, Christine; Auzoux-bordenave, Stephanie.
The decline of European abalone Haliotis tuberculata populations has been associated with various pathogens including bacteria of the genus Vibrio. Following the summer mortality outbreaks reported in France between 1998 and 2000, Vibrio harveyi strains were isolated from moribund abalones, allowing in vivo and in vitro studies on the interactions between abalone H. tuberculata and V. harveyi. This work reports the development of primary cell cultures from abalone gill tissue, a target tissue for bacterial colonisation, and their use for in vitro study of host cell—V. harveyi interactions. Gill cells originated from four-day-old explant primary cultures were successfully sub-cultured in multi-well plates and maintained in vitro for up to 24 days....
Tipo: Text Palavras-chave: Haliotis tuberculata; Vibrio harveyi; Gill cell culture; Pathogenicity; Phenoloxidase; Phagocytosis.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00146/25683/23745.pdf
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De novo assembly and annotation of the European abalone Haliotis tuberculata transcriptome ArchiMer
Harney, Ewan; Dubief, Bruno; Boudry, Pierre; Basuyaux, Olivier; Schilhabel, Markus B.; Huchette, Sylvain; Paillard, Christine; Nunes, Flavia.
The European abalone Haliotis tuberculata is a delicacy and consequently a commercially valuable gastropod species. Aquaculture production and wild populations are subjected to multiple climate-associated stressors and anthropogenic pressures, including rising sea-surface temperatures, ocean acidification and an emerging pathogenic Vibrio infection. Transcript expression data provides a valuable resource for understanding abalone responses to variation in the biotic and abiotic environment. To generate an extensive transcriptome, we performed next-generation sequencing of RNA on larvae exposed to temperature and pH variation and on haemolymph of adults from two wild populations after experimental infection with Vibrio harveyi. We obtained more than 1.5...
Tipo: Text Palavras-chave: RNA-Seq; Mollusca; Gastropoda; Development; Vibrio harveyi; Climate change.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00318/42940/45103.pdf
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Development of TaqMan real-time PCR assays for monitoring Vibrio harveyi infection and a plasmid harbored by virulent strains in European abalone Haliotis tuberculata aquaculture ArchiMer
Schikorski, David; Renault, Tristan; Paillard, Christine; Bidault-toffin, A.; Tourbiez, Delphine; Saulnier, Denis.
The Gram-negative bacterium Vibrio harveyi is known to be highly pathogenic for the European abalone Haliotis tuberculata, which is a gastronomically important marine gastropod with a high commercial value. Since 1998, some particular bacterial strains are described as implicated in recurrent mortality outbreaks in French farm and field stocks of abalone. Recently, a 9.6kb plasmid named pVCR1, was shown to be harbored by one highly V. harveyi virulent ORM4 strain suggesting its involvement in virulence phenotype. Thus, we have developed in the present study two TaqMan real-time PCR assays allowing to (i) rapidly and specifically detect, by a duplex procedure and in less than 2 h, both V. harveyi and the presence of plasmid pVCR1 from unidentified bacterial...
Tipo: Text Palavras-chave: Vibrio harveyi; Haliotis tuberculata; Real-time PCR; Plasmid; Marine pathogen; Molecular diagnostic.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00124/23540/21383.pdf
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Diseases of cultured molluscs in Australia ArchiMer
Lester, R.
Three mollusc species are cultured commercially in Australian tropical and warm temperate waters: the Sydney rock oyster Saccostrea commercialis , the pearl rock oyster is susceptible to two protozoans: Marteilia sydneyi , which is the cause of QX Disease and infects oysters during the summer; and Mikrocytos roughleyi which is associated with winter mortality. It also harbours mudworm, Polydora websteri a parasite farmers try to avoid by growing oysters on racks. Transported pearl oysters Pinctada maxima die with heavy infections of Vibrio harveyi . Cultured giant clams Tridacna gigas are pestered by pyramidellid snails and some carry Perkinsus sp., a protozoan common in reef bivalves. Deaths in mollusc hatcheries have been associated with Vibrio tubiashi...
Tipo: Text Palavras-chave: Protozoa; Alteromonas; Vibrio tubiashi; Perkinsus; Vibrio harveyi; Polydora websteri; Mikrocytos roughleyi; Saccostrea commercialis; Pinctada maxima; Tridacna gigas; Bivalvia; Parasites; Mortality; Pearl culture; Infectious diseases; Parasitic diseases.
Ano: 1989 URL: http://archimer.ifremer.fr/doc/1989/acte-1468.pdf
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Ecophysiological studies on luminous bacteria associated with marine gastropods. ArchiMer
Ramesh, A; Venugopalan, V.
Information available on the association of luminous bacteria with marine invertebrates is limited. Hence qualitative and quantitative investigations were undertaken with a view to understand the distribution of luminous microflora in relation to the environmental factors and also in the gut of two marine gastropods, Aplysia benedicti Eliot and Bullia tranquebarica (Roding) from Porto Novo (11 degree 29'N, 79 degree 46'E) waters of the East coast of India. The gut of the gastropods harboured maximum number of luminous bacteria when compared to the water and sediment. Qualitative analysis revealed the presence of two luminous bacterial components, viz. Vibrio harveyi and V. fishceri. V. fischeri was not found in association with A. benedicti . The isolates...
Tipo: Text Palavras-chave: Gastropoda; Vibrio fischeri; Vibrio harveyi; Bioluminescence; Bacteria; Physicochemical properties; Environmental factors; Microorganisms; Luminous organisms; Interspecific relationships; Ecophysiology.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-993.pdf
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Efecto de Lactobacillus sp. aislado de col fermentada, sobre el peso y los marcadores inmunológicos del camarón blanco Litopenaeus vannamei Rev Salud Anim.
Sánchez Ortiz,Ileana; Martín Martín,Leonardo; García Varela,Yonarky; Abad Márquez,Zoila; Rodríguez,Ramón Franco; Ramírez Núñez,Yamilka; Zamora Sánchez,Jesús; Basulto Morales,Rosa; Moreira Rubio,Alain; Arenal Cruz,Amílcar.
El empleo de probióticos para el control de enfermedades en la acuicultura intensiva es una alternativa al uso de antibióticos. En este trabajo se aisló la cepa bacteriana IC1 a partir de una fermentación acido láctica de col y se identificó por técnicas moleculares y convencionales como Lactobacillus sp. Se determinó la actividad antagónica in vitro de IC1 contra cepas patrones. Para la evaluación de la actividad probiótica un grupo de postlarvas de Litopenaeus vannamei se trató cada 24 horas con 1-2 x10(4) ufc/ml de IC1 y otro se mantuvo sin tratamiento. A los 25 días se cuantificaron el peso húmedo y el comportamiento de los parámetros inmunológicos (actividad de las enzimas superóxido dismutasa, fenoloxidasa y peroxidasa). El peso de los animales que...
Tipo: Journal article Palavras-chave: Probiótico; Litopenaeus vannamei; Vibrio harveyi; Lactobacillus sp.; Fermentaciones vegetales; Bacterias ácido-lácticas.
Ano: 2013 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2013000200004
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Effect of combined function of temperature and water activity on the growth of Vibrio harveyi BJM
Zhou,Kang; Gui,Meng; Li,Pinglan; Xing,Shaohua; Cui,Tingting; Peng,Zhaohui.
Vibrio harveyi is considered as a causative agent of the systemic disease, vibriosis, which occurs in many biological fields. The effects of temperatures (12.9-27.1 ºC) and water activity (NaCl% 0.6%-3.4%) on V. harveyi were investigated. The behavior and growth characteristics of V. harveyi was studied and modeled. Growth curves were fitted by using Gompertz and Baranyi models, and the Baranyi model showed a better fittness. Then, the maximum growth rates (µmax) and lag phase durations (LPD, λ) obtained from both Gompertz and Baranyi model were modeled as a combination function of temperature and water activity using the response surface and Arrhenius-Davey models for secondary model. The value of r², MSE, bias and accuracy factor suggest Baranyi model...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Vibrio harveyi; Modelling; Temperature; Water activity.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400018
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Immune priming and portal of entry effectors improve response to vibrio infection in a resistant population of the European abalone ArchiMer
Dubief, Bruno; Nunes, Flavia; Basuyaux, Olivier; Paillard, Christine.
Since 1997, populations of the European abalone Haliotis tuberculata suffer mass mortalities attributed to the bacterium Vibrio harveyi. These mortalities occur at the spawning season, when the abalone immune system is depressed, and when temperatures exceed 17 °C, leading to favorable conditions for V. harveyi proliferation. In order to identify mechanisms of disease resistance, experimental successive infections were carried out on two geographically distinct populations: one that has suffered recurrent mortalities (Saint-Malo) and one that has not been impacted by the disease (Molène). Furthermore, abalone surviving these two successive bacterial challenges and uninfected abalone were used for several post-infection analyses. The Saint-Malo population...
Tipo: Text Palavras-chave: Immunity; Hemocyte; Abalone; Disease; Extracellular products; Immune priming; Vibrio harveyi; Flow cytometry; Resistance; Phagocytosis; Bacterial growth; QPCR; Gill.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00357/46800/46667.pdf
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Interaction de la bactérie Vibrio harveyi avec son hôte, l'hormeau Haliotis tuberculata : approches physiologiques, cellulaires et moléculaires ArchiMer
Travers, Marie-agnes.
Abalone aquaculture only recently developed in western Europe, but already it has to face numerous pathologies. Since 1998, the natural and farm stocks of Haliotis tuberculata suffer regularly high mortalities, mainly due to the pathogenic bacterium, Vibrio harveyi. The vibriosis of the European abalone, in particular its macroscopic signs, and the conditions necessary for its development have been studied in detail by field and laboratory experiments. We have been able to show the importance of the interplay between water temperature (>17°C), animal physiology (reproduction period) and a pathogenic strain (harbouring the plasmid pVCR1) for the development of this abalone disease. A limited localisation study of the abalone target tissues (gills, muscle...
Tipo: Text Palavras-chave: Ormeau; Haliotis tuberculata; Bactéries; Vibrio harveyi; Vibriose; Température; Immunité; Reproduction; Hémocytes; MAP Kinases; Plasmide; Virulence; GFP; Abalone; Haliotis tuberculata; Bacteria; Vibrio harveyi; Vibriosis; Temperature; Immunity; Reproduction; Haemocytes; MAP Kinases; Plasmid; Virulence; GFP.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/00176/28729/27196.pdf
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Molecular variations in Vibrio alginolyticus and V. harveyi in shrimp-farming systems upon stress BJM
Santhyia,Anix Vivek; Mulloorpeedikayil,Rosalind George; Kollanoor,Riji John; Jeyaseelan,Prince M.J..
A study was performed to investigate the genomic variations in the shrimp farm isolates of Vibrio alginolyticus and V. harveyi when the isolates were subjected to environmental stress. Samples of shrimps, water and sediment were collected from Southern Indian coastal shrimp farms. Vibrio isolates were biochemically identified and confirmed using 16S rDNA and gyrB gene specific PCR. The bacterial strains were genotyped by PCR fingerprinting using GTG(5) and IS (Insertion Sequence) primers. Seven strains each of V. alginolyticus and V. harveyi were subjected to 10 passages through trypticase soya broth (TSB), which contained different NaCl concentrations (3, 6 and 8%) and trypticase soya agar (TSA). V. alginolyticus was also passaged through TSB with a 12%...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Vibrio harveyi; Vibrio alginolyticus; Molecular variation; Shrimp; Penaeus monodon.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401001
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Quorum sensing in Enterococcus faecium, Enterococcus faecalis and Bacillus cereus strains isolated from ricotta processing Ciência Rural
Fernandes,Meg da Silva; Esper,Luciana Maria Ramires; Kabuki,Dirce Yorika; Kuaye,Arnaldo Yoshiteru.
ABSTRACT: The quorum sensing phenomenon is a process of intra- and inter-species microbial communication involving the production and detection of extracellular signaling molecules. The autoinducer AI-2 has been proposed to serve as a ‘universal signal’ for interspecies communication. This study aimed to evaluate the capability of Enterococcus faecium, Enterococcus faecalis, and Bacillus cereus strains isolated from ricotta processing to produce quorum sensing signalling molecules (AI-2). The strains were evaluated for the presence of the luxS gene using the polymerase chain reaction. AI-2 quorum sensing signalling molecules were measured in relative light units (RLUs) using a luminometer. A total of 74% of E. faecium, 91% of E. faecalis, and 95% of B....
Tipo: Info:eu-repo/semantics/article Palavras-chave: LuxS; AI-2; Vibrio harveyi; Bioluminescence; Enterococcus spp.; Bacillus cereus.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000200751
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Quorum sensing inhibitors from Leucetta chagosensis Dendy, 1863 ArchiMer
Mai, T.; Tintillier, Florent; Lucasson, Aude; Moriou, C.; Bonno, E.; Petek, S.; Magre, Kevin; Al Mourabit, A.; Saulnier, Denis; Debitus, C..
Sponges are a rich source for investigation of bioactive small molecules. They have been mostly investigated for the search of new pharmacological models or therapeutic agents for the treatment of human diseases. Micro-organisms can also represent a virulent pathogen for marine invertebrates such as sponges, which need to protect themselves against these microbes. Sponges' self defence mechanisms involving dialogue molecules thus represent a pertinent research track for potent anti-infective and anti-biofilm activities such as quorum sensing inhibitors (QSIs). The investigation of the QSI crude extract of Leucetta chagosensis Dendy, 1863 led to the isolation of three new alkaloids, isonaamine D, di-isonaamidine A and leucettamine D, along with the known...
Tipo: Text Palavras-chave: Biofouling; Leucetta chagosensis; Leucettamine; Naamidine; Naamine; Quorum sensing; Quorum sensing inhibitors; Sponges; Vibrio harveyi.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00275/38597/37189.pdf
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Seasonal variation in the antivibrio activity of two organic extracts from two red seaweed: Palmaria palmata and the introduced Grateloupia turuturu against the abalone pathogen Vibrio harveyi ArchiMer
Garcia-bueno, Nuria; Dumay, Justine; Guerin, Thomas; Turpin, Vincent; Paillard, Christine; Stiger-pouvreau, Valerie; Pouchus, Yves-francois; Aitor Marin-atucha, Arnaldo; Decottignies, Priscilla; Fleurence, Joel.
The wide polarity range and highly polar compounds of two selected red seaweed, Grateloupia turuturu and Palmaria palmata were extracted using two different types of solvent, dichloromethane/methanol and methanol/water. Monthly in vitro antibacterial activities were studied using the microplate method against the marine bacteria Vibrio harveyi strain ORM4, known to infect abalone. Inhibition, slowdown and delay of Vibrio harveyi growth were investigated. Polar compounds of seaweed showed an activity against the abalone pathogen. The best activity was recorded from P. palmata collected in spring, with an inhibition of 7.9% of the bacterial growth. Preliminary 1H NMR profiles identified the differences between the extracts.
Tipo: Text Palavras-chave: Antibacterial activity; Abalone disease; Haliotis tuberculata; Red seaweed; Vibrio harveyi.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00617/72875/71929.pdf
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Time-related action of Lactobacillus plantarum in the bacterial microbiota of shrimp digestive tract and its action as immunostimulant. Repositório Alice
VIEIRA, F. do N.; BUGLIONE NETO, C.C.; MOURIÑO, J.L.P.; JATOBÁ, A.; RAMIREZ, C.; MARTINS, M.L.; BARRACCO, M.A.A.M.; VINATEA, L.A..
The objective of this work was to assess the time-related action of probiotic Lactobacillus plantarum in the bacterial microbiota of the digestive tract of Litopenaeus vannamei, and the relation of total haemocyte count and serum phenol oxidase activity of shrimp challenged with Vibrio harveyi. Shrimps were fed with a probiotic-supplemented diet, for eight days, then shifted to a commercial diet. Shrimps fed only with the commercial diet served as control. Evaluations were made on the 8th day of experiment and repeated two, four, six and eight days later. Total lactic bacteria in the digestive tract was higher until the 4th day of evaluation in the probiotic-supplemented group. Vibrio spp. counts were higher in the control at days zero and two. Until the...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Litopenaeus vannamei; Vibrio harveyi; Total haemocyte count; Lactic bacteria; Phenol oxidase; Probiotic; Contagem total de hemócitos; Bactérias lácticas; Fenoloxidase; Probiótico.
Ano: 2008 URL: http://www.alice.cnptia.embrapa.br/handle/doc/112076
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Time-related action of Lactobacillus plantarum in the bacterial microbiota of shrimp digestive tract and its action as immunostimulant PAB
Vieira,Felipe do Nascimento; Buglione Neto,Celso Carlos; Mouriño,José Luiz Pedreira; Jatobá,Adolfo; Ramirez,Cristina; Martins,Maurício Laterça; Barracco,Margherita Anna Antonia Maria; Vinatea,Luis Alejandro.
The objective of this work was to assess the time-related action of probiotic Lactobacillus plantarum in the bacterial microbiota of the digestive tract of Litopenaeus vannamei, and the relation of total haemocyte count and serum phenol oxidase activity of shrimp challenged with Vibrio harveyi. Shrimps were fed with a probiotic-supplemented diet, for eight days, then shifted to a commercial diet. Shrimps fed only with the commercial diet served as control. Evaluations were made on the 8th day of experiment and repeated two, four, six and eight days later. Total lactic bacteria in the digestive tract was higher until the 4th day of evaluation in the probiotic-supplemented group. Vibrio spp. counts were higher in the control at days zero and two. Until the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Litopenaeus vannamei; Vibrio harveyi; Total haemocyte count; Lactic bacteria; Phenol oxidase; Probiotic.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000600013
Registros recuperados: 15
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