|
|
|
|
|
| Asinari,F; Cafrune,E.E; Guzmán,F.A; Conci,L.R; Conci,V.C. |
| The vegetative propagation of strawberries favors transmission of systemic pathogens, such as viruses, which are one of the main yield-limiting factors for this crop. More than 20 viruses have been described as infecting this species; one of the most frequent is the Strawberry mottle virus (SMoV), which is responsible for significant economic losses. SMoV is usually detected by reverse transcription polymerase chain reaction (RT-PCR), given that serum is not available for serological tests. In this study, a non-radioactive molecular probe was developed for SMoV detection. The cDNA was synthesized by RT-PCR using specific primers designed from the 3’UTR region of the viral genome. The cloned cDNA segment was labeled and used as a probe. Six RNA... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fragaria x ananassa; Molecular techniques; Screening viruses; Virus diagnosis. |
| Ano: 2016 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-298X2016000100004 |
| |
|
|
| Mujica,María Valentina; Mora,Roxana; Rosales,Marlene; Sandoval,Claudio. |
| A vineyard in south-central Chile was surveyed during five growing seasons to analyze the increase over time in the number of plants infected with Grapevine leafroll-associated virus-1, -2 and -3. At the end of the study, GLRaV-3 was found to be the most prevalent virus, with 46% of plants infected by this pathogen. GLRaV-2 infected 13% of plants, and GLRaV-1 infected 12%. Considering all three viruses together, the prevalence of infection increased exponentially from 14% of the plants at the beginning of the study period to 68% at the end. The patterns of the spread of GLRaV-2 and GLRaV-3 were mostly along the rows, whereas the pattern of spread for GLRaV-1 could not be determined. |
| Tipo: Journal article |
Palavras-chave: Field transmission; Grapevine leafroll-associated virus; GLRaV-1; GLRaV-2; GLRaV-3; Virus diagnosis. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202013000100012 |
| |
|
|
|
