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Assessment of Antimicrobial Activity and Healing Potential of Mucous Secretion of Achatina fulica International Journal of Morphology
Santana,Wagno Alcântara; Melo,Cláudia Moura de; Cardoso,Juliana Cordeiro; Pereira-Filho,Rose Nely; Rabelo,Alessandra Silva; Reis,Francisco Prado; Albuquerque-Júnior,Ricardo Luiz Cavalcanti de.
Achatina fulica's secretion has been recently related to antibacterial, antifungal, and cicatricial properties, and it is influenced by the kind of food offered. Therefore, this study investigated the healing potential of dressing films based on mucous secretion of Achatina fulica. Thus, surgical wounds performed in black wistar rats were dressed with films based on collagen, and on mucous secretion of A. fulica fed with Lactuca sativa; undresses worked as wounds control. After 3, 7, 14 and 21 days the animals were euthanized, and the wounds were microscopically evaluated. On the 3rd and 7th days dressing films based on mucus provided acceleration of the formation maturation of granulation tissue, better epithelization rates, and more rapid replacement of...
Tipo: Journal article Palavras-chave: Wound healing; Wound dressing; Land snails; Mucus.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000200001
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Comparison of the Efficacy of Honey and Animal Oil in Accelerating Healing of Full Thickness Wound of mice skin International Journal of Morphology
Ghaderi,Reza; Afshar,Mohammad; Akhbarie,Hadi; Golalipour,Mohammad Jafar.
There are some medications and procedures that can be used to accelerate the healing of skin wounds. Some studies have demonstrated improvement of burn wound healing with honey treatment. In other hand, based on traditional medicine have improved wound healing with animal oil. This study was done to compare the efficacy of animal oil and honey in accelerating healing of full thickness wound of skin in mice. In this experimental study 36 male NMRI mice were subjected to full-thickness skin wounds under general anesthesia. Animals were randomly allocated to receive either single daily applications of placebo, animal oil and honey (n=12 for each group) respectively. On 4th, 7th and 10th days, four mice from each group were sacrificed using an overdose of...
Tipo: Journal article Palavras-chave: Honey; Animal oil; Wound healing; Skin; Mice.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000100027
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Control of the skin scarring response Anais da ABC (AABC)
Ferreira,Lydia M.; Gragnani,Alfredo; Furtado,Fabianne; Hochman,Bernardo.
There comes a time when the understanding of the cutaneous healing process becomes essential due to the need for a precocious tissue repair to reduce the physical, social, and psychological morbidity. Advances in the knowledge on the control of interaction among cells, matrix and growth factors will provide more information on the Regenerative Medicine, an emerging area of research in medical bioengineering. However, considering the dynamism and complexity of the cutaneous healing response, it is fundamental to understand the control mechanism exerted by the interaction and synergism of both systems, cutaneous nervous and central nervous, via hypothalamus hypophysis-adrenal axis, a relevant subject, but hardly ever explored. The present study reviews the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Wound healing; Keratinocytes; Fibroblasts; Keloid; Tissue engineering; Skin; Plastic surgery.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000300024
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Copaiba oil in experimental wound healing in horses Ciência Rural
Lucas,Flavia de Almeida; Kandrotas,Adriana Lima; Nardin Neto,Eugenio; Siqueira,Carlos Eduardo de; André,Gabriela Santo; Bromerschenkel,Ingrid; Perri,Silvia Helena Venturoli.
ABSTRACT: The aim of this study was to evaluate the effects of 10% copaiba oil in experimentally induced wounds in horses. Four wounds were made in the lumbar and metacarpal regions of eight adult horses. In the treatment group, the wounds received 10% copaiba oil and in the control group 0.9% sodium chloride, in the daily dressing for 21 days. The wounds were evaluated three, 7, 14, and 21 days postoperatively. No significant differences were observed between the groups. The mean lumbar wound contraction rates were 80.54% and 69.64%, for the control and treated groups, respectively. For the wounds in the metacarpal region, these averages were 44.15% and 52.48%, respectively. Under the experimental conditions of the present study, it is concluded that 10%...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phytotherapy; Wound healing; Equine.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000400601
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Co-transfection of hepatocyte growth factor and truncated TGF-β type II receptor inhibit scar formation BJMBR
Xu,Ji-Hua; Zhao,Wan-Yi; Fang,Qing-Qing; Wang,Xiao-Feng; Zhang,Ding-Ding; Hu,Yan-Yan; Zheng,Bin; Tan,Wei-Qiang.
Wound scarring remains a major challenge for plastic surgeons. Transforming growth factor (TGF)-β plays a key role in the process of scar formation. Previous studies have demonstrated that truncated TGF-β type II receptor (t-TGF-βRII) is unable to continue signal transduction but is still capable of binding to TGF-β, thereby blocking the TGF-β signaling pathway. Hepatocyte growth factor (HGF) is a multifunctional growth factor that promotes tissue regeneration and wound healing. Theoretically, the combination of HGF and t-TGF-βRII would be expected to exert a synergistic effect on promoting wound healing and reducing collagen formation. In the present study, lentivirus-mediated transfection of the two genes (t-TGF-βRII/HGF) into fibroblasts in vitro and in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Wound healing; Truncated transforming growth factor-β type II receptor; Hepatocyte growth factor; Scar; Rat; Lentivirus.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000100611
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Effect of Bovine Type-I Collagen-Based Films Containing Red Propolis on Dermal Wound Healing in Rodent Model International Journal of Morphology
Albuquerque-Júnior,Ricardo Luiz Cavalcanti de; Barreto,André Luiz Santos; Pires,Jônatas Almeida; Reis,Francisco Prado; Lima,Sônia Oliveira; Ribeiro,Maria Amália Gonzaga; Cardoso,Juliana Cordeiro.
Propolis, an apitherapic product widely employed in natural medicine, presents various biological properties, such as bactericidal and anti-inflammatory activities, but only few reports have looked at the Brazilian red propolis variety. Collagen films have been employed to improve wound healing and some studies have investigated the effect of natural products incorporated into these films. Thus, the aim of this study was to analyze the effect of collagen-based films containing red propolis on wound healing. Therefore, wounds performed in the back of rats were dressed with collagen-based films (G2), and collagen-based films containing red propolis (G3); undressed worked as control (G1). After 3, 7 and 14 days the animals were euthanized, and the wounds were...
Tipo: Journal article Palavras-chave: Propolis; Wound healing; Occlusive dressings; Type-I collagen.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000400025
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Effect of sodium carboxymethylcellulose and methylprednisolone on the healing of jejunal anastomoses in rats BJMBR
Sousa,A.A.; Petroianu,A.; Trapiello Neto,V.; Rios,V.S.; Barbosa,A.J.A..
Sodium carboxymethylcellulose (SCMC) has been effective in reducing adhesion formation and corticosteroids reduce the inflammatory process. The objective of this study was to define the intraperitoneal (ip) effects of SCMC combined with intramuscular (im) methylprednisolone on peritoneal adhesion formation and on jejunal anastomosis healing in rats. Twenty Wistar rats (200-350 g) were divided into four groups (N = 5): groups I and III (controls) 5 and 21 days of treatment before sacrifice, respectively; groups II and IV (experimental groups) 5 and 21 days of treatment, respectively. SCMC (1%) was infused into the abdominal cavity and methylprednisolone (10 mg kg-1 day-1) was injected im daily from the day before surgery for animals of groups II and IV. All...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bowel anastomosis; Peritoneal adhesions; Sodium carboxymethylcellulose; Corticosteroid; Histology; Wound healing.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000400012
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Effects of Green Tea Use on Wound Healing International Journal of Morphology
Neves,Ana Luiza de Almeida; Komesu,Marilena Chinali; Di Matteo,Miguel Angel Sala.
Green tea, a product from Camellia sinensis, consists of over 200 componentes. The most known are the catechins, ou polyphenolic compounds, or flavonoids, but it contains also polysacharide conjugates, amino acids, caffeine and vitamins. Studies have suggested that the regular consumption of green tea reduces the risk of cancer, protecting agains initial events and progression of the disease, may act as antioxidant, has bactericidal properties, and that green tea consumption may be an adjuvant in the treatment of diabetes mellitus, hypertension and hypercholesterolemia. Our experimental study was carried out with male Wistar rats, separated in two groups, experimental and control. The animals in control group were given water ad libitum, and the animals in...
Tipo: Journal article Palavras-chave: Green tea; Wound healing; Wistar Rat.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000300039
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Effects of strain and age on ear wound healing and regeneration in mice BJMBR
Costa,R.A.; Ruiz-de-Souza,V.; Azevedo Jr.,G.M.; Vaz,N.M.; Carvalho,C.R..
Round holes in the ears of MRL mice tend to close with characteristics of regeneration believed to be absent in other mouse strains (e.g., C57BL/6). We evaluated the kinetics and the histopathology of ear wound closure in young (8 weeks old) C57BL/6 and BALB/c mice. We also used middle-aged (40 weeks old) C57BL/6 mice to evaluate the influence of aging on this process. A circular through-and-through hole was made in the ear, photographs were taken at different times after injury and wound area was measured with digital analysis software. The percentages of closed area measured on day 100 were: 23.57 ± 8.66% for young BALB/c mice, 56.47 ± 7.39% for young C57BL/6 mice, and 75.31 ± 23.65% for middle-aged C57BL/6 mice. Mice were sacrificed on days 1, 3, 5, 25,...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Wound healing; Regeneration; Scar; Aging; C57BL/6 mice; BALB/c mice.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200004
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Effects of vacuum sealing drainage on the treatment of cranial bone-exposed wounds in rabbits BJMBR
Chen,X.J.; Liu,S.; Gao,G.Z.; Yan,D.X.; Jiang,W.S..
This study was designed to assess the efficacy of vacuum sealing drainage (VSD) on skull exposure wounds in rabbits and to investigate the underlying mechanism of the process. Full-thickness excisional circular wounds 2×2 cm with or without periosteum involvement were created in 88 New Zealand white rabbits (mean body weight: 3.0±0.65 kg). Animals were randomly divided into 4 groups: periosteum-intact wounds treated with traditional dressing (p+control), periosteum-intact wounds treated with VSD (p+VSD), periosteum-lacking wounds treated with traditional dressing (p–control) and periosteum-lacking wounds treated with VSD (p–VSD). The wounds treated with traditional dressing were covered with Vaseline gauze, while VSD treatment was accompanied with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cranial bone-exposed wound; Vacuum sealing drainage; Periosteum; Wound healing; Efficacy.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001200602
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Enhancement of cellular activity in hyperglycemic mice dermal wounds dressed with chitosan-alginate membranes BJMBR
Breder,J.S.C.; Pires,A.L.R.; Azevedo,F.F.; Apolinário,P.P.; Cantaruti,T.; Jiwani,S.I.; Moraes,Â.M.; Consonni,S.R.; Araújo,E.P.; Adams,G.G.; Saad,M.J.A.; Lima,M.H.M..
The use of specially designed wound dressings could be an important alternative to facilitate the healing process of wounds in the hyperglycemic state. Biocompatible dressings combining chitosan and alginate can speed up wound healing by modulating the inflammatory phase, stimulating fibroblast proliferation, and aiding in remodeling phases. However, this biomaterial has not yet been explored in chronic and acute lesions of diabetic patients. The aim of this study was to evaluate the effect of topical treatment with a chitosan-alginate membrane on acute skin wounds of hyperglycemic mice. Diabetes mellitus was induced by streptozotocin (60 mg · kg-1 · day-1 for 5 days, intraperitoneally) and the cutaneous wound was performed by removing the epidermis using...
Tipo: Info:eu-repo/semantics/article Palavras-chave: C57BL/6J mice; Wound healing; Wound dressing; Polysaccharides; Diabetes.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000100602
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Evaluation of cytotoxicity and wound healing activity of Avicennia schaueriana in cream Anais da ABC (AABC)
LOPES,CAROLINE M.I.; BARATELLA-EVÊNCIO,LIRIANE; SOUZA,IVONE A. DE; OLIVEIRA,ERWELLY B. DE; SÁ,JÉSSICA G.A.; SANTANA,MARLLON A.N.; MARCELINO NETO,PEDRO P.; SANTANA,EDUARDA S. DE; SILVA,LUZIA A. DA; VIEIRA,JEYMESSON R.C..
Abstract:Avicennia schaueriana is found in Brazilian mangrove coast. The cytotoxicity in vitro of the Aqueous Extract of Leaves of Avicennia schaueriana (AELAs) and the healing activity of the plant in cream on mice skin wounds were evaluated in this study. The cytotoxic evaluation was performed on Vero cells. The healing activity was evaluated on mice treated during 5, 10 and 15 days with cream at 5%, solution of sodium chloride at 0.9% and dexpanthenol in cream at 5%. The extract did not show cytotoxicity, but showed mitogenic activity (100μg/ml). In morphometric analysis, the percentage of wound contraction after 10 days was higher in dexpanthenol group (93.41%). In 15 days, the lowest percentage of contraction was observed in the dexpanthenol group...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Avicennia schaueriana; In vitro techniques; Re-epithelialization; Vero cells; Wound healing.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000100805
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Evaluation of the Effect of Platelet-Rich Plasma on Alveolar Wound Healing in Rats International Journal of Morphology
Yamamoto,Marcos Kazuo; Novelli,Moacyr Domingos; Corrêa,Luciana; de Sousa,Suzana Cantanhede M; Luz,João Gualberto C.
Opinions about the clinical utility of platelet-rich plasma (PRP) vary, as a large number of experimental studies have questioned its efficacy. The purpose of this study was to evaluate the effects of PRP on experimental alveolar wound healing in rats. Fifty young adult male Wistar rats were divided in control and PRP groups and submitted to extraction of the right maxillary incisor. In the PRP group, blood was collected by cardiac puncture, and the socket was filled with a PRP gel. Animals were euthanized after 1, 3, 7, 14 and 30 days. Histological and histomorphometric analyses were performed at each experimental time point. Semiquantitative histological analysis showed that the PRP group exhibited significantly more collagen-matrix deposition and less...
Tipo: Journal article Palavras-chave: Tooth socket; Wound healing; Platelet-rich plasma; Animal experimentation.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000100041
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Evaluation of wound healing and antimicrobial properties of aqueous extract from Bowdichia virgilioides stem barks in mice Anais da ABC (AABC)
AGRA,ISABELA K.R.; PIRES,LUANA L.S.; CARVALHO,PAULO S.M.; SILVA-FILHO,EURÍPEDES A.; SMANIOTTO,SALETE; BARRETO,EMILIANO.
The decoction of the stem barks from Bowdichia virgilioides KUNTH is a folk remedy used to treat inflammatory disorders in Latin American and Brazil. In the present study, the wound healing activity of aqueous extract of the stem bark from B. virgilioides, called AEBv, was evaluated by the rate of healing by wound contraction and period of epithelization at different days post-wound using the wound excisional model. On day 9, the AEBv-treated animals exhibited significative reduction in the wound area when compared with controls. In wound infected with S. aureus, the AEBv significantly improved the wound contraction when compared to the saline-treated mice. The histological analysis showed that AEBv induced a collagen deposition, increase in the fibroblast...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Wound healing; Antimicrobial effect; Bowdichia virgilioides; Medicinal plant.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000300945
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Exploring the in vivo wound healing effects of a recombinant hemolin from the caterpillar Lonomia obliqua J. Venom. Anim. Toxins incl. Trop. Dis.
Sato,Ana Claudia; Bosch,Rosemary Viola; Will,Sonia Elisabete Alves; Alvarez-Flores,Miryam Paola; Goldfeder,Mauricio Barbugiani; Pasqualoto,Kerly Fernanda Mesquita; Silva,Bárbara Athayde Vaz Galvão da; Andrade,Sonia Aparecida de; Chudzinski-Tavassi,Ana Marisa.
Abstract Background Hemolin proteins are cell adhesion molecules from lepidopterans involved in a wide range of cell interactions concerning their adhesion properties. However, hemolin’s roles in cell proliferation and wound healing are not fully elucidated. It has been recently reported that rLosac, a recombinant hemolin from the caterpillar Lonomia obliqua, presents antiapoptotic activity and is capable of improving in vitro wound healing. Therefore, this study aimed to explore rLosac’s in vivo effects using a skin wound healing model in rats. Methods Circular full-thickness wounds in the rat dorsum skin were treated either with rLosac, or with saline (control), allowing healing by keeping the wounds occluded and moist. During the wound healing, the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hemolin; RLosac; Wound healing; Collagen.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100324
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Histopathological Effects of Ischemia - Reperfusion on Intestinal Anastomotic Wound Healing in Dogs International Journal of Morphology
Akinrinmade,J. F; Olatunji-Akioye,A. O.
The histopathological effects of three levels of ischemia-reperfusion (IR) on healing of intestinal anastomotic wound were investigated in dogs. Three groups of dogs in which superior mesenteric artery (SMA) and collaterals were isolated but not occluded (A -control), isolated and occluded immediately distal to the aorta with collateral interruption using an atraumatic arterial clip for fifteen minutes (B) and for forty-five minutes (C) prior to ileal resection and anastomosis after return of normal perfusión in occluded groups was done. Within each group, animals were anaesthesized on third and seventh post-operative day. Grossly, impaired abdominal wound healing, intra-abdominal adhesions and partial and complete anastomotic dehiscence occurred in groups...
Tipo: Journal article Palavras-chave: Histopathology; Ischemia-reperfusion injury; Intestinal anastomoses; Wound healing.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000400020
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Immune Cells Depletion During Wound Healing as a Long-Term Effect of Undernutrition International Journal of Morphology
Zuanassi Macari,Marcelo; Misael Cavenaghi,Fabiano; Chinali Komesu,Marilena; Orive Lunardi,Laurelúcia; Sala,Miguel Angel; Novaes Júnior,Arthur Belém; Grisi,Márcio Fernando de Moraes; Taba Júnior,Mário; Scombatti de Souza,Sérgio Luiz.
Undernutrition in early life is associated with a number of acute and chronic sequelae, and recovering is a controversial issue. Even if undernutrition in Brazil is declining, studies have shown that about 31% of brazilian children still present severe or moderate malnutrition. The present study goal was to induce early malnutrition in rats and observe short- (undernourished) and long-term (after recovered) effects on defense cells involved in wound healing. Undernutrition was produced by separating the pups from the mother for 10 hours/day during the suckling period (21 days after birth). As controls were used rats at same age not submitted to suckling restriction. Undernutrition and recovering states were assessed by body weight. Skin wounds were made on...
Tipo: Journal article Palavras-chave: Undernourishment; Wound healing; Defense cells.
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022005000100005
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Intradermal injection of Bothrops cotiara venom in mice in an experimental wound model J. Venom. Anim. Toxins incl. Trop. Dis.
Lopes,JA; Giménez,APL; Zischler,LFCM; Stuelp-Campelo,PM; Moreno,AN; Elifio-Esposito,SL.
Bothropic envenomation induces hemorrhage, coagulant disturbances and necrosis. Regarding therapies against the local damage caused by the venom, there is little information on tissue changes until the complete healing. In the current study, local damage was evaluated by examination of morphological inflammatory alterations, mast cell count, and analysis of collagen deposition. Bleeding was evident four hours after inoculation. After 24 hours, a large area of injury appeared presenting disorganized tissue, significant hemorrhage and acute inflammation. After three days, the damaged area was extensive, with a large amount of inflammatory cells and the presence of scab. In seven days, healing and reepithelization process started. And, 21 days later, the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bothrops venom; Wound healing; Inflammation; Local damage.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100009
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Involvement of mast cells, CD68+ and VEGF+ expressions in response to Himatanthus drasticus commercial latex in mice wound healing model Arq. Bras. Med. Vet. Zootec.
Santos,G.J.L.; Ferreira,T.C.; Rodrigues,A.L.M.; Freitas,J.C.C.; Morais,S.M.; Girão,V.C.C.; Nunes-Pinheiro,D.C.S..
ABSTRACT This study aimed to evaluate Himatanthus drasticus latex in a mice wound healing experimental model. Animals were divided into four groups (n=7) according to the treatments: GI - saline 0.9% (control), GII - mineral oil (vehicle), GIII - H. drasticus commercial latex (HdCL) and GIV - H. drasticus mixed isolated fraction (MIF, 1 mg/mL). The treatments were applied topically once daily, 50 µL for 14 consecutive days. Macroscopic lesions were evaluated, considering parameters such as swelling, redness, granulation tissue and reepithelialization. VEGF+, CD68+ expressions and mast cells (Toluidin blue stain) were evaluated. HdCL induced higher contraction and exuberant granulation tissue (P > 0.05). HdCL showed a mild inflammatory process while MIF...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Latex; Apocinaceae; Wound healing; Angiogenesis; Inflammatory infiltrate.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000300513
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Moderate intensity physical training accelerates healing of full-thickness wounds in mice BJMBR
Zogaib,F.G.; Monte-Alto-Costa,A..
Physical training influences the cells and mediators involved in skin wound healing. The objective of this study was to determine the changes induced by different intensities of physical training in mouse skin wound healing. Ninety male C57BL6 mice (8 weeks old, 20-25 g) were randomized into three physical training groups: moderate (70% VO2max), high (80% VO2max), and strenuous intensity (90% VO2max). Animals trained on a motorized treadmill for 8 weeks (Elesion: physical training until the day of excisional lesion, N = 10) or 10 weeks (Eeuthan: physical training for 2 additional weeks after excisional lesion until euthanasia, N = 10), five times/week, for 45 min. Control groups (CG) trained on the treadmill three times/week only for 5 min (N = 10). In the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Physical training; Wound healing; Myofibroblast; Skin.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001000008
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