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Genetic analysis of D-xylose metabolism by endophytic yeast strains of Rhodotorula graminis and Rhodotorula mucilaginosa Genet. Mol. Biol.
Xu,Ping; Bura,Renata; Doty,Sharon L..
Two novel endophytic yeast strains, WP1 and PTD3, isolated from within the stems of poplar (Populus) trees, were genetically characterized with respect to their xylose metabolism genes. These two strains, belonging to the species Rhodotorula graminis and R. mucilaginosa, respectively, utilize both hexose and pentose sugars, including the common plant pentose sugar, D-xylose. The xylose reductase (XYL1) and xylitol dehydrogenase (XYL2) genes were cloned and characterized. The derived amino acid sequences of xylose reductase (XR) and xylose dehydrogenase (XDH) were 32%~41% homologous to those of Pichia stipitis and Candida. spp., two species known to utilize xylose. The derived XR and XDH sequences of WP1 and PTD3 had higher homology (73% and 69% identity)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rhodotorula graminis; Rhodotorula mucilaginosa; D-xylose metabolism; Xylitol; Xylitol dehydrogenase; Xylose reductase.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300018
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Obtaining partial purified xylose reductase from Candida guilliermondii BJM
Tomotani,Ester Junko; Arruda,Priscila Vaz de; Vitolo,Michele; Felipe,Maria das Graças de Almeida.
The enzymatic bioconversion of xylose into xylitol by xylose reductase (XR) is an alternative for chemical and microbiological processes. The partial purified XR was obtained by using the following three procedures: an agarose column, a membrane reactor or an Amicon Ultra-15 50K Centrifugal Filter device at yields of 40%, 7% and 67%, respectively.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Xylose reductase; Xylitol; Partial purification.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300027
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