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Analysis of the interaction between human kidney anion exchanger 1 and kanadaptin using yeast two-hybrid systems 74
Wongthida,Phonphimon; Akkarapatumwong,Varaporn; Limjindaporn,Thawornchai; Kittanakom,Saranya; Keskanokwong,Thitima; Eurwilaichitr,Lily; Yenchitsomanus,Pa-thai.
Kidney anion exchanger adaptor protein (Kanadaptin) is a protein which interacts with the cytoplasmic N-terminal domain of kidney anion exchanger 1 (kAE1) and was first detected in mice using the yeast two-hybrid system and was also found to co-localize with kAE1 in rabbit a-intercalated cells. Impaired trafficking of human kAE1 can result in the kidney disease-distal renal tubular acidosis (dRTA), and defective interaction between human kAE1 and kanadaptin may cause this trafficking impairment and be the basis for dRTA pathogenesis. However, it is unknown whether kAE1 can really interact with kanadaptin in humans. We have thus investigated the interaction between human kAE1 and human kanadaptin by using both Gal4 and LexA yeast two-hybrid systems. It was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Kidney anion exchanger 1; Kanadaptin; Yeast two-hybrid system.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100003
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Neurospora crassa mat A-2 and mat A-3 proteins weakly interact in the yeast two-hybrid system and affect yeast growth 74
Silva,Carla C. da; Cruz,Rosana C.; Bucciarelli-Rodriguez,Mônica; Vilas-Boas,Adlane.
Mating-type genes control the entry into the sexual cycle, mating identity and sexual development in fungi. The mat A-2 and mat A-3 genes, present in the mat A idiomorph of the filamentous fungus Neurospora crassa, are required for post-fertilization functions but are not essential for mating identity. Their putative roles as transcription factors are based on the similarity of mat A-2 with the Podospora anserina SMR1 gene and an HMG motif present in the mat A-3 gene. In this work the yeast two-hybrid system was used to identify transcriptional activity and protein-protein interaction of N. crassa mat A-2 and mat A-3 genes. We observed that the mat A-3 protein alone is capable of weakly activating transcription of yeast reporter genes; it also binds with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Neurospora crassa; Mating-type; Yeast two-hybrid system.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200023
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The 42-kDa coat protein of Andean potato mottle virus acts as a transcriptional activator in yeast 56
Vidal,M.S.; Margis,R..
Interactions of viral proteins play an important role in the virus life cycle, especially in capsid assembly. Andean potato mottle comovirus (APMoV) is a plant RNA virus with a virion formed by two coat proteins (CP42 and CP22). Both APMoV coat protein open reading frames were cloned into pGBT9 and pGAD10, two-hybrid system vectors. HF7c yeast cells transformed with the p9CP42 construct grew on yeast dropout selection media lacking tryptophan and histidine. Clones also exhibited ß-galactosidase activity in both qualitative and quantitative assays. These results suggest that CP42 protein contains an amino acid motif able to activate transcription of His3 and lacZ reporter genes in Saccharomyces cerevisiae. Several deletions of the CP42 gene were cloned into...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Andean potato mottle virus; Virus assembly; Coat protein; Transactivation; Yeast two-hybrid system.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400001
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