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Registros recuperados: 5
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Cell envelope components of Yersinia pestis grown in intraperitoneal diffusion chambers Rev. Microbiol.
Ferreira,Rita C. C.; Neto,Armando A. B.; Fracalanzza,Sérgio E. L.; Costa,Sérgio O. P.; Almeida,D. F.; Ferreira,Luís C. S..
The electrophoretic profiles of penicillin binding proteins (PBPs) and outer membrane proteins (OMPs) of Yersinia pestis EV 76 were determined following in vivo growth in diffusion chambers implanted in the peritoneal cavity of mice. In contrast to Y. pestis grown under in vitro conditions which activate the low calcium response (LCR) regulon there was no significant qualitative or quantitative change of the PBP profile of Y. pestis cells during growth in diffusion chambers for up to 72 h following implantation in mice. Three OMPs, with molecular weight of 100, 60 and 58 kDa, were expressed in Y. pestis cells grown for 24 h, but not at 48 h or at 72 h, in diffusion chambers. These results indicate that growth of Y. pestis in intraperitoneal diffusion...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Yersinia pestis; PBP; In vivo growth; OMP.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000300005
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Humoral and cellular immune response of mice challenged with Yersinia pestis antigenic preparations BJID
Leal,Elida A.; Moreira,Josimar D.; Nunes,Fernanda F.; Souza,Larissa R.; Martins,Janaina M.; Toledo,Vicente P.C.; Almeida,Alzira M.P.; Guimarães,Tania M.P..
ABSTRACT Objectives: The plague, which is an infectious disease caused by Yersinia pestis, still threatens many populations in several countries. The worldwide increase in human plague cases and the potential use of the bacteria as a biological weapon reinforce the need to study the immunity that is induced by potential vaccine candidates. To determine the immunogenicity of antigenic preparations based on the F1 protein and the total extract from Y. pestis, we assessed the role of these antigens in inducing an immune response. Methods: The immunogenicity of antigenic preparations based on the Y. pestis (YP) total extract and the Y. pestis fraction 1 capsular antigen protein (F1) was determined in Swiss-Webster mice immunized with 40 µg or 20 µg for each...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Yersinia pestis; Antigens; Mice; Immune response; Vaccines.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000600620
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Ongoing quiescence in the Borborema Plateau Plague focus (Paraiba, Brazil) Anais da ABC (AABC)
ZEPPELINI,CAIO G.; DE ALMEIDA,ALZIRA M.P.; CORDEIRO-ESTRELA,PEDRO.
Abstract Plague is a zoonosis caused by Yersinia pestis, whose cycle is based on a reservoir system composed of mammals and their fleas. Its transmission cycle presents long enzootic periods with undetected cases, sometimes misleading that the cycle is extinct. While surveillance activities in Brazil are being carried out only in some focal areas, the serologic results confirm the persistence of Y. pestis in all monitored areas. We studied the small mammal assembly and Y. pestis presencein the Borborema Plateau Focus within the state of Paraíba, which staged the last Brazilian plague outbreak (1986-1987), through aninventory and Y. pestis detection survey of small mammals in peridomestic and sylvatic areas from two municipalities in the state of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Small mammals; Peridomestic environment; Yersinia pestis; Quiescence; Surveillance.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000603007
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Polysiloxane/PVA-glutaraldehyde hybrid composite as solid phase for immunodetections by ELISA BJMBR
Lima Barros,A.E.; Almeida,A.M.P.; Carvalho Jr.,L.B.; Azevedo,W.M..
We developed an efficient method to prepare a hybrid inorganic-organic composite based on polyvinyl alcohol (PVA) and polysiloxane using the sol-gel disc technique. Antigen obtained from Yersinia pestis was covalently immobilized onto these discs with glutaraldehyde and used as solid phase in ELISA for antibody detection in serum of rabbits experimentally immunized with plague. Using 1.25 µg antigen per disc, a peroxidase conjugate dilution of 1:4,000 and a serum dilution of 1:200 were adequate for the establishment of the procedure. These values are similar to those used for PVA-glutaraldehyde discs, plasticized filter paper discs and the polyaniline-Dacron composite discs. This procedure is comparable to that which utilizes the adsorption of the antigen...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Polysiloxane; Polyvinyl alcohol; Solid phase; Immunodetection; Yersinia pestis; ELISA.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400008
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Yersinia pestis detection by loop-mediated isothermal amplification combined with magnetic bead capture of DNA BJM
Feng,Na; Zhou,Yazhou; Fan,Yanxiao; Bi,Yujing; Yang,Ruifu; Zhou,Yusen; Wang,Xiaoyi.
ABSTRACT We developed a loop-mediated isothermal amplification (LAMP) assay for the detection of Y. pestis by targeting the 3a sequence on chromosome. All 11 species of the genus Yersinia were used to evaluate the specificity of LAMP and PCR, demonstrating that the primers had a high level of specificity. The sensitivity of LAMP or PCR was 2.3 or 23 CFU for pure culture, whereas 2.3 × 104 or 2.3 × 106 CFU for simulated spleen and lung samples. For simulated liver samples, the sensitivity of LAMP was 2.3 × 106 CFU, but PCR was negative at the level of 2.3 × 107 CFU. After simulated spleen and lung samples were treated with magnetic beads, the sensitivity of LAMP or PCR was 2.3 × 103 or 2.3 × 106 CFU, whereas 2.3 × 105 or 2.3 × 107 CFU for magnetic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Plague; Yersinia pestis; Loop-mediated isothermal amplification; Magnetic beads.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100128
Registros recuperados: 5
Primeira ... 1 ... Última
 

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