Data/hora: 12/06/2024 17:21:50

Provedor de dados: | 1
País: | Brazil
Título: | Ultrastructural and cytochemical analysis of physic nut callus tissue in response to different combinations of growth regulators
Autores: | Santos, Dalilhia Nazaré dos; Nunes, Claudinéia Ferreira; Soares, Joyce Dória Rodrigues; Alves, Eduardo; Labory, Cláudia Regina Contijo; Pasqual, Moacir; Pio, Leila Aparecida Salles.
Data: | 2015-08-03
Ano: | 2015
Palavras-chave: | Jatropha curcas L.; Embryogenesis; Microscopy; Cytochemistry.
Resumo: | This study aimed to induce callus formation in Jatropha curcas L. and to evaluate the ultrastructure and cytochemical behavior of the calli. Calluses were induced with 2,4-D, picloram-PIC, kinetin (Kin) and BAP: (1) control; (2) 4.52 µM 2,4-D; (3) 9.04 µM 2,4-D; (4) 4.14 µM PIC; (5) 8.28 µM PIC; (6) 4.52 µM 2,4-D + 2.32 µM KIN; (7) 9.04 µM 2,4-D + 4.64 µM KIN; (8) 4.14 µM PIC + 2.32 µM KIN; (9) 8.28 µM PIC + 4.64 µM KIN; (10) 4.52 µM 2,4-D + 2.22 µM BAP; (11) 9.04 µM 2,4-D + 4.44 µM BAP; (12) 4.14 µM PIC + 2.22 µM BAP and (13) 8.28 µM PIC + 4.44 µM BAP. It was evaluated the percent coverage of the explants by callus (% CEC) and performed scanning electron microscopy (SEM) and acetocarmine/Evans blue double staining to analyze the embryogenic potential of the calli. As shown by scanning electron microscopy and acetocarmine/Evans blue staining, we found that J. curcas callus formation was optimal with 4.52 µM of 2,4-D.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês

Identificador:

http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19745

10.4025/actasciagron.v37i3.19745

Editor: | Universidade Estadual de Maringá
Relação: | http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19745/pdf_86
Formato: | application/pdf

Fonte:

Acta Scientiarum. Agronomy; v. 37, n. 3 (2015); 355-359

Acta Scientiarum. Agronomy; v. 37, n. 3 (2015); 355-359

1807-8621

1679-9275