Data/hora: 09/06/2024 12:02:57

Provedor de dados: | 102
País: | Brazil
Título: | Tomato chlorotic spot virus in hydroponically-grown lettuce in São Paulo State, Brazil
Autores: | Colariccio,Addolorata; Eiras,Marcelo; Chaves,Alexandre L. R.; Harakava,Ricardo; Chagas,César M.
Data: | 2004-06-01
Ano: | 2004
Palavras-chave: | Tospovirus; Serology; RT-PCR; Sequencing.
Resumo: | In the regions of Campinas and Sumaré, São Paulo, Brazil, hidroponically grown crops of Lettuce (Lactuca sativa) cv. Verônica, which showed virus-like symptoms were examined by electron microscope, biological, serological and molecular tests. Pleomorphic, enveloped particles (80-100 nm in diameter) were always detected in these samples. Experimentally inoculated host plants, including lettuce, reacted with tospoviruses-induced symptoms. Some differences were observed in Gomphrena globosa, which reacted by showing local lesions and systemic mosaic. Two isolates of Tomato chlorotic spot virus (TCSV) were identified by DAS-ELISA and by RT-PCR. The sequencing and alignment of the RT-PCR coat protein amplified fragments have indicated a high degree of homology with the TCSV sequences stored in the GenBank. This is the first report of losses due to a virus from the genus Tospovirus in commercial hydroponic lettuce crops in Brazil. Further epidemiological studies are needed for better understanding the spread of the virus in hydroponic crops, since Tomato spotted wilt virus (TSWV) is reported to spread through the nutritive solution.
Tipo: | Info:eu-repo/semantics/other
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582004000300012
Editor: | Sociedade Brasileira de Fitopatologia
Relação: | 10.1590/S0100-41582004000300012
Formato: | text/html
Fonte: | Fitopatologia Brasileira  v.29 n.3 2004
Fonte: | info:eu-repo/semantics/openAccess