Data/hora: 14/06/2024 11:16:25

Provedor de dados: 	102	
País: 	Brazil	
Título: 	Cytopathology of callus cells infected with grapevine leafroll-associated virus 3	
Autores: 	SCAGLIUSI,SANDRA M. M.; VEGA,JORGE; KUNIYUKI,HUGO.	
Data: 	2002-07-01	
Ano: 	2002	
Palavras-chave: 	Closterovirus; Vitis spp.; Transmission electron microscopy.	
Resumo: 	The cytopathology of grapevine (Vitis spp.) callus tissue infected with Grapevine leafroll-associated virus 3 (GLRaV-3), genus Vitivirus was studied in order to investigate the usefulness of callus cultures to study grapevine leafroll-associated viruses. Ultrathin sections were made from in vitro callus obtained from stems and shoots of GLRaV-3 infected grapevine plants. Callus was composed of two types of tissue. Translucent, soft callus was formed and composed of large loosely arranged cells, containing big vacuoles and a thin layer of cytoplasm. Other parts of the callus were brown-coloured and composed of small compactly arranged cells, which showed flexuous and rod-shaped closterovirus-like particles, with 10-12 nm in diameter, at higher magnifications. Groups of vesicles formed by a single membrane were also observed, with sizes ranging from 50-200 nm, containing fine fibrillar material, also typical of closterovirus infections. Virus concentration was monitored by Immunosorbent electron microscopy (ISEM) tests, which showed that in vitro culture of callus tissue from grapevine infected plants, could be used to study the GLRaV viruses through many successive generations, despite the decline in virus concentration after repeated transfers. No virus particles were observed in callus tissue obtained from healthy grapevines.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582002000400008	
Editor: 	Sociedade Brasileira de Fitopatologia	
Relação: 	10.1590/S0100-41582002000400008	
Formato: 	text/html	
Fonte: 	Fitopatologia Brasileira  v.27 n.4 2002	
Fonte: 	info:eu-repo/semantics/openAccess