Data/hora: 06/06/2024 14:11:48

Provedor de dados: | 102
País: | Brazil
Título: | Use of semi-seletive media for detection of Sclerotinia sclerotiorum on bean and soybean seeds
Autores: | PERES,ÂNGELA P.; NASSER,LUIZ CARLOS B.; MACHADO,JOSÉ DA CRUZ.
Data: | 2002-04-01
Ano: | 2002
Palavras-chave: | Seed health tests; Fungus; Phaseolus vulgaris; Glycine max.
Resumo: | This work was aimed at evaluating the possibility of using bromophenol blue as an indicator for detecting the presence of Sclerotinia sclerotiorum in the seeds of dry-beans (Phaseolus vulgaris) and soybean (Glycine max), through incubation of the seeds on an agar medium and "blotter" substrates. The seeds were artificially inoculated with four S. sclerotiorum isolates, plated on the agar medium, named Neon, and on modified Neon agar media all incubated at 14 and 20 ºC for seven days in the dark. Half of the seeds inoculated were surface desinfested prior to plating on the medium. The seeds showing change of colour in the medium, from blue to light yellow, as well as formation of typical mycelium and sclerotia in some cases, were considered to be infected or contaminated by S. sclerotiorum. The two incubation temperatures compared did not show significant (P&lt;0.05) differences in detection level for most of the isolates tested on the different media. According to results obtained in this study, the Neon agar medium with incubation at 14 or 20 ºC has proved to be a reliable and quick method for the detection of S. sclerotiorum mycelium in naturally infected seeds of bean and soybean.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582002000200001
Editor: | Sociedade Brasileira de Fitopatologia
Relação: | 10.1590/S0100-41582002000200001
Formato: | text/html
Fonte: | Fitopatologia Brasileira  v.27 n.2 2002
Fonte: | info:eu-repo/semantics/openAccess