Data/hora: 06/06/2024 08:11:17

Provedor de dados: | 102
País: | Brazil
Título: | Genetic diversity of begomoviruses infecting soybean, bean and associated weeds in Northwestern Argentina
Autores: | Rodríguez-Pardina,Patricia E.; Zerbini,F. Murilo; Ducasse,Daniel A.
Data: | 2006-08-01
Ano: | 2006
Palavras-chave: | Sida mottle virus; Bean golden mosaic virus; Geminivirus.
Resumo: | The subtropical Northwestern region of Argentina (provinces of Tucumán, Salta, Jujuy, Santiago del Estero and Catamarca) suffers from a high incidence of the whitefly Bemisia tabaci, and the detection of begomoviruses is also common. The Northwest is the main bean-growing region of the country, and approximately 10% of Argentina's soybean crop is grown in this area. We have used a PCR-based assay to establish the identity and genetic diversity of begomoviruses associated with bean and soybean crops in Northwestern Argentina. Universal begomovirus primers were used to direct the amplification of a fragment encompassing the 5' portion of the capsid protein gene. Amplified fragments were cloned, sequenced and subjected to phylogenetic analysis to determine the sequence identity to known begomoviruses. The data indicated the presence of four distinct begomoviruses, all related to other New World begomoviruses. The prevalent virus, which was present in 94% of bean and soybean samples and also in two weed species, is closely related to Sida mottle virus (SiMoV). A virus with high sequence identity with Bean golden mosaic virus (BGMV) was found in beans. The two remaining viruses displayed less than 89% identity with other known begomoviruses, indicating that they may constitute novel species. One of these putative novel viruses was detected in bean, soybean and tomato samples.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582006000400003
Editor: | Sociedade Brasileira de Fitopatologia
Relação: | 10.1590/S0100-41582006000400003
Formato: | text/html
Fonte: | Fitopatologia Brasileira  v.31 n.4 2006
Fonte: | info:eu-repo/semantics/openAccess