Data/hora: 16/06/2024 19:57:07

Provedor de dados: 	102	
País: 	Brazil	
Título: 	Comparação da seqüência de nucleotídeos do gene da proteína capsidial de estirpes severas e premunizantes do Papaya ringspot virus	
Autores: 	Della Vecchia,Marilia G. S.; Camargo,Luis E. A.; Rezende,Jorge A. M.	
Data: 	2003-12-01	
Ano: 	2003	
Palavras-chave: 	Cross protection; Cucurbits; Molecular virology.	
Resumo: 	This study compared three mild and three severe strains of Papaya ringspot virus - type W (PRSV-W), based on nucleotide and amino acid sequences of the capsid protein (CP) gene. The CP nucleotide sequences of the mild strains shared 98% to 100% identity. When compared to the severe strains the identity ranged from 93% to 95%, except in the case of PRSV-W-2R, which resulted from reversion of the mild strains PRSV-W-2. The CP sequence of the reverting strain showed 100% identity with the sequence of its parental strain. An insertion of six nucleotides in the core region of the CP gene, which reflected the addition of two amino acids (Asn and Asp) in the deduced sequence of the protein, was found in all mild strains. These sequence comparisons were used to design strain-specific primers that were used to specifically amplify regions for either the mild or severe strains.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582003000600015	
Editor: 	Sociedade Brasileira de Fitopatologia	
Relação: 	10.1590/S0100-41582003000600015	
Formato: 	text/html	
Fonte: 	Fitopatologia Brasileira  v.28 n.6 2003	
Fonte: 	info:eu-repo/semantics/openAccess