Data/hora: 09/06/2024 12:43:15

Provedor de dados: | 102
País: | Brazil
Título: | Sequences of the coat protein gene from brazilian isolates of Papaya ringspot virus
Autores: | LIMA,ROBERTO C. A.; SOUZA JR.,MANOEL T.; PIO-RIBEIRO,GILVAN; LIMA,J. ALBERSIO A.
Data: | 2002-04-01
Ano: | 2002
Palavras-chave: | Potyvirus; Carica papaya; PRSV; Phylogenetic analysis.
Resumo: | Papaya ringspot virus (PRSV) is the causal agent of the main papaya (Carica papaya) disease in the world. Brazil is currently the world's main papaya grower, responsible for about 40% of the worldwide production. Resistance to PRSV on transgenic plants expressing the PRSV coat protein (cp) gene was shown to be dependent on the sequence homology between the cp transgene expressed in the plant genome and the cp gene from the incoming virus, in an isolate-specific fashion. Therefore, knowledge of the degree of homology among the cp genes from distinct PRSV isolates which are present in a given area is important to guide the development of transgenic papaya for the control of PRSV in that area. The objective of the present study was to assess the degree of homology among the PRSV cp genes of several Brazilian isolates of this virus. Papaya and PRSV are present in many different ecosystems within Brazil. Twelve PRSV isolates, collected in eight different states from four different geographic regions, were used in this study. The sequences of the cp gene from these isolates were compared among themselves and to the gene used to generate transgenic papaya for Brazil. An average degree of homology of 97.3% at the nucleotide sequence was found among the Brazilian isolates. When compared to 27 isolates from outside Brazil in a homology tree, the Brazilian isolates were clustered with Australian, Hawaiian, and Central and North American isolates, with an average degree of homology of 90.7% among them.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582002000200009
Editor: | Sociedade Brasileira de Fitopatologia
Relação: | 10.1590/S0100-41582002000200009
Formato: | text/html
Fonte: | Fitopatologia Brasileira  v.27 n.2 2002
Fonte: | info:eu-repo/semantics/openAccess