Data/hora: 09/06/2024 13:27:49

Provedor de dados: 	102	
País: 	Brazil	
Título: 	Molecular and pathogenic variability of Drechslera isolates from oats	
Autores: 	MEHTA,Y. R.	
Data: 	2001-09-01	
Ano: 	2001	
Palavras-chave: 	Pyrenophora avenae; Avena sativa; Molecular characterization; Varietal resistance.	
Resumo: 	Severe epidemics of leaf blotch and black leaf spot of oat (Avena sativa) caused by Drechslera avenae and Drechslera sp., respectively, are frequently observed in the State of Paraná, Brazil. Although some morphological differences between the isolates causing two different symptoms were noticed, the genetic relationship between them was not clear. Twenty-four isolates of D. avenae and Drechslera sp, collected between 1996-98, were assessed for the genetic variability by molecular and pathogenic analyses. The amplification products using primer pair ITS4/ITS5 showed a fragment length of approximately 600 bp for all the isolates except for one black spot isolate, where the fragment length was approximately 550 bp. Restriction enzymes Hinf I and Taq I, that cut in the ITS region, produced similar restriction patterns for all the isolates, whereas four others produced variable restriction patterns. RAPD analysis also showed distinctive patterns for some isolates. No clear difference between the black spot and the leaf blotch isolates was observed either by the molecular or by the pathogenicity analysis. Nonetheless, the rDNA analysis suggests that Drechslera probably comprises at least three distinct taxa. The results indicate that the difference observed between the isolates originating from two types of symptoms is due to intra-specific variants of D. avenae.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582001000300002	
Editor: 	Sociedade Brasileira de Fitopatologia	
Relação: 	10.1590/S0100-41582001000300002	
Formato: 	text/html	
Fonte: 	Fitopatologia Brasileira  v.26 n.3 2001	
Fonte: 	info:eu-repo/semantics/openAccess