Data/hora: 02/06/2024 02:47:38

Provedor de dados: 	103	
País: 	Brazil	
Título: 	Insecticide resistance and genetic variability in natural populations of Aedes (Stegomyia) aegypti (Diptera: Culicidae) from Colombia	
Autores: 	Aguirre-Obando,Oscar A.; Bona,Ana C. Dalla; Duque L.,Jonny E.; Navarro-Silva,Mário A.	
Data: 	2015-02-01	
Ano: 	2015	
Palavras-chave: 	Bioassays; Gene flow; Knockdown resistance; Mitochondrial DNA; ND4 gene.	
Resumo: 	Mosquito control prevails as the most efficient method to protect humans from the dengue virus, despite recent efforts to find a vaccine for this disease. We evaluated insecticide resistance and genetic variability in natural populations of Aedes aegypti (Linnaeus, 1762) from Colombia. This is the first Colombian study examining kdr mutations and population structure. Bioassays with larvae of three mosquito populations (Armenia, Calarcá and Montenegro) were performed according to the World Health Organization (WHO) guidelines, using Temephos. For the analysis of the Val1016Ile mutation and genetic diversity, we sampled recently-emerged adults from four mosquito populations (Armenia, Calarcá, Montenegro and Barcelona). Following the WHO protocol, bioassays implemented with larvae showed resistance to Temephos in mosquito populations from Armenia (77% ± 2) and Calarcá (62% ± 14), and an incipient altered susceptibility at Montenegro (88% ± 8). The RR95 of mosquito populations ranged from 3.7 (Montenegro) to 6.0 (Calarca). The Val1016Ile mutation analysis of 107 genotyped samples indicates that 94% of the specimens were homozygous for the wild allele (1016Val) and 6% were heterozygous (Val1016Ile). The 1016Ile allele was not found in Barcelona. Genetic variability analysis found three mitochondrial lineages with low genetic diversity and gene flow. In comparison with haplotypes from the American continent, those from this study suggest connections with Mexican and North American populations. These results confirm that a continuous monitoring and managing program of A. aegypti resistance in the state of Quindío is required.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702015000100014	
Editor: 	Sociedade Brasileira de Zoologia	
Relação: 	10.1590/S1984-46702015000100003	
Formato: 	text/html	
Fonte: 	Zoologia (Curitiba)  v.32 n.1 2015	
Fonte: 	info:eu-repo/semantics/openAccess