Data/hora: 12/06/2024 09:32:44

Provedor de dados: | 115
País: | Mexico
Título: | An improved method for in vitro regeneration of common bean (Phaseolus vulgaris L.)
Autores: | Quintero-Jiménez,Anareli; Espinosa-Huerta,Elsa; Acosta-Gallegos,J. Alberto; Guzmán-Maldonado,H. Salvador; Mora-Avilés,M. Alejandra.
Data: | 2010-02-01
Ano: | 2010
Palavras-chave: | Adenine; Bud clusters; Embryonic axes; Gamborg medium; Organogenesis.
Resumo: | In vitro regeneration of common bean (Phaseolus vulgaris L.) is a requirement for genetic transformation which involves induction and development to the whole plant. Success in regeneration of common bean from tissue organ culture has been achieved to some extent in the last years. However, genotype effects in regeneration response as well as efficiency and reproducibility have been a limiting factor. Embryos, from four different varieties, were excised from sterilized mature seeds and cultured in Murashige and Skoog (MS) or Gamborg (GM) media containing 6-benzylaminopurine (BAP) (10 mg-1) and adenine (A) (0 or 20 mg-1). Efficient regeneration was achieved when inducing formation of differentiation of cells like bud clusters at the internodal segment of the embryo axes in the four varieties studied. One way analysis of variance and Tukey media comparison (p≤0.05) showed that regeneration efficiency varied considerably between the two basic media. GM media provided high bud cluster formation (97.8 to 100 %) and full plant regeneration (93 %), whereas MS medium showed lower bud cluster formation (15 to 73 %), and full plant regeneration (29 %). It is provided evidence of how the culture media and growth regulators influence the regeneration of common bean, when seeking for an efficient transformation protocol.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952010000100005
Editor: | Colegio de Postgraduados
Formato: | text/html
Fonte: | Agrociencia  v.44 n.1 2010
Fonte: | info:eu-repo/semantics/openAccess