Data/hora: 05/06/2024 12:47:40

Provedor de dados: | 118
País: | Chile
Título: | Effect of the inoculum size on the axenic in vitro production of Steinernema carpocapsae
Autores: | Suárez-Sánchez,Juan; Alatorre-Rosas,Raquel; Poggi-Varaldo,Héctor M; Barrera-Cortés,Josefina.
Data: | 2012-04-01
Ano: | 2012
Palavras-chave: | Axenic culture; Hemoglobin; In vitro culture; Steinernema carpocapsae; Transparent medium.
Resumo: | The effect of inoculum size on the axenic in vitro production of Steinernema carpocapsae, using a transparent medium with hemoglobin and cholesterol, was studied. Axenic cultivation was performed with fertile eggs ofS. carpocapsae (hatching of eggs: 89 ± 4%), which were obtained by lyses of female nematodes and subsequent incubation. Two important aspects of this work include the use of a completely transparent medium enriched with hemoglobin and cholesterol and the high multiplication factor of the population (MFIP) of 87, which is considered high for in vitro axenic cultures of nematodes. In vivo methods have the advantage of high MFIP, but they also have the disadvantage of the high number and complexity of the operations involved for the purposes of harvesting axenic nematodes. It is assumed that the availability of a high number of nematodes in a transparent medium will further encourage the characterization of new native strains of nematodes.
Tipo: | Journal article
Idioma: | Inglês
Identificador: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000100011
Editor: | Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal
Formato: | text/html
Fonte: | Ciencia e investigación agraria  v.39 n.1 2012