Data/hora: 16/06/2024 00:46:41

Provedor de dados: | 17
País: | United Kingdom
Título: | SC1/PRDM4 recruits PRMT5 to control the timing of neural precursor differentiation in developing neural stem cells
Autores: | Alexandra Chittka; Ursula Grazini.
Data: | 2010-09-20
Ano: | 2010
Palavras-chave: | Molecular Cell Biology; Neuroscience.
Resumo: | During cortical development, neural stem cells (NSCs) switch from proliferative to neuron-generating asymmetric divisions. Here we investigated the role of Schwann cell factor 1 (SC1/PRDM4), a transcriptional repressor highly expressed in the developing nervous system, during NSCs development. We found that SC1 protein levels were down-regulated in newly differentiating neurons, while remaining high in undifferentiated NSCs, suggesting an asymmetric inheritance of SC1. Knockdown of SC1 in the NSCs led to precocious differentiation of neurons and its overexpression led to an increase in Nestin-expressing precursors. We found that SC1, through its amino-terminus, recruits the chromatin modifier PRMT5, a histone arginine methyltransferase that catalyses histone H4R3 symmetric dimethylation (H4R3me2s). Mutations disrupting SC1/PRMT5 interaction resulted in loss of SC1-mediated increase in undifferentiated neural precursor cells. Our data demonstrate that SC1 and PRMT5 are components of an epigenetic regulatory complex that provides an epigenetic signature of a &amp;#x201c;stem-like&amp;#x201d; cellular state in the NSCs and which may be asymmetrically inherited during neurogenic divisions.
Tipo: | Poster

Identificador:

http://precedings.nature.com/documents/4926/version/1

oai:nature.com:10.1038/npre.2010.4926.1

http://dx.doi.org/10.1038/npre.2010.4926.1

Fonte: | Nature Precedings
Fonte: | Creative Commons Attribution 3.0 License