Data/hora: 03/06/2024 10:55:34

Provedor de dados: | 17
País: | United Kingdom
Título: | Genome sequence and genetic linkage analysis of Shiitake mushroom _Lentinula edodes_
Autores: | Hoi Shan Kwan; Chun Hang Au; Man Chun Wong; Jing Qin; Iris Sze Wai Kwok; Winnie Wing Yan Chum; Pui Ying Yip; Kin Sing Wong; Lei Li; Qian Li Huang; Wen Yan Nong.
Data: | 2012-01-31
Ano: | 2012
Palavras-chave: | Genetics &amp; Genomics.
Resumo: | _Lentinula edodes_ (Shiitake/Xianggu) is an important cultivated mushroom. Understanding the genomics and functional genomics of _L. edodes_ allows us to improve its cultivation and quality. Genome sequence is a key to develop molecular genetic markers for breeding and genetic manipulation. We sequenced the genome of _L. edodes_ monokaryon L54A using Roche 454 and ABI SOLiD genome sequencing. Sequencing reads of about 1400Mb were de novo assembled into a 40.2 Mb genome sequence. We compiled the genome sequence into a searchable database with which we have been annotating the genes and analyzing the metabolic pathways. In addition, we have been using many molecular techniques to analyze genes differentially expressed during development. Gene ortholog groups of _L. edodes_ genome sequence compared across genomes of several fungi including mushrooms identified gene families unique to mushroom-forming fungi. We used a mapping population of haploid basidiospores of dikaryon L54 for genetic linkage analysis. High-quality variations such as single nucleotide polymorphisms, insertions, and deletions of the mapping population formed a high-density genetic linkage map. We compared the linkage map to the _L. edodes_ L54A genome sequence and located selected quantitative trait loci. The Shiitake community will benefit from these resources for genetic studies and breeding.&amp;#xd;&amp;#xa;
Tipo: | Poster

Identificador:

http://precedings.nature.com/documents/6855/version/1

oai:nature.com:10.1038/npre.2012.6855.1

http://dx.doi.org/10.1038/npre.2012.6855.1

Fonte: | Nature Precedings
Fonte: | Creative Commons Attribution 3.0 License