Data/hora: 05/06/2024 13:23:15

Provedor de dados: | 17
País: | United Kingdom
Título: | Interleukin-10 containing normal human serum inhibits granzyme B release but not perforin release from alloreactive and EBV-specific T cell clones
Autores: | Motoko Nishimura; Hideya Sato; Hitoshi Okazaki; Masahiro Satake; Kenji Tadokoro.
Data: | 2007-12-05
Ano: | 2007
Palavras-chave: | Immunology.
Resumo: | Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor, has pleiotropic effects in immunoregulation and inflammation. It is capable of inhibiting synthesis of pro-inflammatory cytokines like interferon [gamma] (IFN[gamma]), IL-2, IL-3, tumor necrosis factor [alpha] (TNF[alpha]) and granulocyte macrophage colony stimulating factor (GM-CSF) made by cells such as macrophages and T helper Type 1 cells. We observed that normal human serum, derived from a healthy individual but containing large amounts of IL-10 (arbitrarily designated as &amp;#x22;IL-10 serum&amp;#x22;), inhibited cytotoxic activity and interfered with granzyme B release from alloreactive cytotoxic T cell clones in vitro, but did not affect perforin release. The addition of normal human serum containing high levels of anti-IL-10 IgG (arbitrarily designated as &amp;#x22;anti-IL-10 IgG serum&amp;#x22;) neutralized the inhibitory effects of IL-10 serum. Moreover, we have identified that cytotoxic activity and granzyme B release from an Epstein-Barr virus (EBV)-specific cytotoxic T cell clone was similarly inhibited in the presence of IL-10 serum, while perforin release was unaffected. Anti-IL-10 IgG serum also appeared to neutralize the inhibitory effect of IL-10 serum on an EBV-specific T cell clone. When anti-IL-10 IgG was depleted from anti-IL-10 IgG containing serum (arbitrarily designated as &amp;#x22;anti-IL10 IgG free serum&amp;#x22;), the neutralizing effect disappeared for both alloreactive and an EBV-specific T cell clone.
Tipo: | Manuscript

Identificador:

http://precedings.nature.com/documents/1390/version/1

oai:nature.com:10101/npre.2007.1390.1

http://hdl.handle.net/10101/npre.2007.1390.1

Fonte: | Nature Precedings
Fonte: | Creative Commons Attribution 3.0 License