Data/hora: 05/06/2024 01:23:56

Provedor de dados: | 17
País: | United Kingdom
Título: | Azadirachta indica induced suppression of Mycobacterium tuberculosis secreted proteins in human monocyte
Autores: | Jawed Iqbal; Ajay Kumar; Najmul Islam.
Data: | 2008-02-01
Ano: | 2008
Palavras-chave: | Microbiology; Molecular Cell Biology.
Resumo: | The H37Rv strain of MTB was grown in modified Sauton&amp;#x2019;s medium till mid-log phase. Peripheral blood mononuclear cells (PBMC) were isolated by density gradient sedimentation on Ficoll-Paque separation medium. Adherent monocytes obtained from PBMC&amp;#x2019;s were infected with H37Rv for 90 min. in the ratio of 1:1 (1 bug: 1 cell). Thereafter, after washing, infected cells were co-cultured with varying doses of neem extract for 24 hrs, and harvested subsequently. Modulation of secreted TNF-[alpha], iNOS and MTB Ag85 complex expressions in culture supernatants was estimated by ELISA.&amp;#xd;&amp;#xa;We report the high basal expression of secreted tumor necrosis factor-[alpha] (TNF-[alpha]), inducible nitric oxide synthase (iNOS) and MTB Ag85 complex in MTB-infected monocytes was suppressed by neem extract in a dose-dependent manner (P&amp;#x3c;0.001 for all). A concentration of 1ug/ml of neem extract showed a suppression by ~ 2.5-fold (P&amp;#x3c;0.001), 1.7-fold (P&amp;#x3c;0.001) and 1.8-fold (P&amp;#x3c;0.001) in the expressions of&amp;#xd;&amp;#xa;secreted TNF-[alpha], iNOS and MTB Ag85 respectively in 24 hr culture supernatants of MTB-infected monocytes.&amp;#xd;&amp;#xa;Thus, neem extract seems to be a potential future adjunct for in-depth studies in the management of tuberculosis.
Tipo: | Poster

Identificador:

http://precedings.nature.com/documents/1562/version/1

oai:nature.com:10.1038/npre.2008.1562.1

http://dx.doi.org/10.1038/npre.2008.1562.1

Fonte: | Nature Precedings
Fonte: | Creative Commons Attribution 3.0 License