Data/hora: 11/06/2024 01:24:51

Provedor de dados: 	17	
País: 	United Kingdom	
Título: 	A Hyperstable Miniprotein: Additive Effects of D- and L-Ala Substitutions	
Autores: 	D. V. Williams; Bipasha Barua; Niels H. Andersen.	
Data: 	2008-07-12	
Ano: 	2008	
Palavras-chave: 	Chemistry.	
Resumo: 	The effects of alanine substitutions in each helical segment of the structure, and Gly to D-Ala mutations at sites where glycines have positive phi angles in the Trp-cage miniprotein are reported. The effects of the stabilizing mutation were additive, yielding a 20-residue construct (Tm = 83^o^C). Gly to L-Ala substitutions were uniformly destabilizing ([DELTA][DELTA]G~F~ &amp;#x3e; 11 kJ/mol): the preference for a D-Ala can be as large as 16 kJ/mol. Glycine to D-Ala mutations are validated as a strategy for the design of hyperstable miniprotein scaffolds suitable for stereospecific pharmacophore display.	
Tipo: 	Manuscript	
Identificador: 	http://precedings.nature.com/documents/2074/version/1

oai:nature.com:10101/npre.2008.2074.1

http://hdl.handle.net/10101/npre.2008.2074.1	
Fonte: 	Nature Precedings	
Fonte: 	Creative Commons Attribution 3.0 License