Data/hora: 06/06/2024 03:26:30

Provedor de dados: | 17
País: | United Kingdom
Título: | Allicin from garlic suppresses TNF-&amp;#x3b1; and augments IFN-&amp;#x3b3; expressions in monocyte cultures from patients with Vaginitis
Autores: | Najmul Islam; Jawed Iqbal; Nazarul Hasan; Zeeshan Fatima; Hamida Thakur; Nazia Hasan; Abbas Ali Mahdi; Tamkin Khan; Farzana Bano; Irfan Ahmad Ansari.
Data: | 2008-02-01
Ano: | 2008
Palavras-chave: | Immunology; Microbiology; Molecular Cell Biology.
Resumo: | Objective: We tried to explore beneficial effects of allicin- a natural antioxidant from garlic in the possible management of vaginitis. &amp;#xd;&amp;#xa;Methods: Peripheral blood mononuclear cells (PBMC&amp;#x2019;s) were isolated from blood of patients having vaginal infections as well as normal healthy subjects as per our published protocol. Monocytes (MN&amp;#x2019;s) from above PBMC&amp;#x2019;s were adhered, rested overnight and cultured without or with varying doses of allicin (0-500 ng/ml). Some cultures received SN50 and SN50M (100 ug/ml). After 24 hrs cultures, the cells were harvested and the supernatants subjected to secreted TNF-&amp;#x3b1; and sIFN-&amp;#x3b3; assays by ELISA. Treated/untreated harvested cells were subjected to glutathione peroxidase (GPx) activity assay.&amp;#xd;&amp;#xa;Results: We show involvement of inflammatory cytokines and reactive oxygen species (ROS) in vaginitis. Augmented secreted TNF-&amp;#x3b1; and decreased sIFN-&amp;#x3b3; levels were observed in monocyte cultures of vaginal patients. Relative to healthy controls, a decrease by 2.5-fold in glutathione peroxidase (GPx) activity in cultures of vaginal patients was observed. Treatment with allicin from garlic appreciably decreased secreted TNF-&amp;#x3b1; with simultaneous amelioration in sIFN-&amp;#x3b3; and GPx activity. Allicin (500 ng/ml) ameliorated GPx activity by ~ 2.16-folds in patient monocyte cultures. The anti-oxidant and anti-inflammatory actions of allicin induced in monocyte cultures of vaginal infection patients were mediated via NF-&amp;#x3ba;B.&amp;#xd;&amp;#xa;Conclusion: Thus, the above results may help in understanding the use of allicin as an adjunct in vaginal infection therapy, and in turn, may address the globally female health.&amp;#xd;&amp;#xa;
Tipo: | Manuscript

Identificador:

http://precedings.nature.com/documents/1560/version/1

oai:nature.com:10.1038/npre.2008.1560.1

http://dx.doi.org/10.1038/npre.2008.1560.1

Fonte: | Nature Precedings
Fonte: | Creative Commons Attribution 3.0 License