Data/hora: 04/06/2024 18:47:54

Provedor de dados: | 17
País: | United Kingdom
Título: | Applying of novel subtracted method Genetically Directed Differential Subtraction Chain (GDDSC) in plant genomes
Autores: | Ewa Siedlecka; Aneta Hromada-Judycka; Magdalena Pawe&amp;#x142;kowicz; Rafa&amp;#x142; W&amp;#xf3;ycicki; Monika Rakoczy-Trojanowska; Zbigniew Przybecki.
Data: | 2010-12-30
Ano: | 2010
Palavras-chave: | Biotechnology; Developmental Biology; Genetics &amp; Genomics; Plant Biology.
Resumo: | We present a simple subtraction procedure of GDDSC as a modification of the original DSC and GDRDA methods. Genetically Directed Differential Subtraction Chain (GDDSC) is a process by which highly related genomes are compared in order to isolate tags carrying the polymorphisms. &amp;#xd;&amp;#xa;To detect specific DNA fragment (tag), we can then monitor offspring plants for efficient molecular breeding. The GDDSC protocol was applied to isolation of new sex related clones from cucumber plants (_Cucumis_ _sativus_ L.) and new tissue culture response clones from rye (_Secale_ _cereale_ L.). The newly identified tags, obtained by GDDSC represent pools of candidate genes and other sequences, which could serve as potential markers for requested traits.
Tipo: | Manuscript

Identificador:

http://precedings.nature.com/documents/5465/version/1

oai:nature.com:10.1038/npre.2010.5465.1

http://dx.doi.org/10.1038/npre.2010.5465.1

Fonte: | Nature Precedings
Fonte: | Creative Commons Attribution 3.0 License