Data/hora: 05/06/2024 05:43:27

Provedor de dados: | 17
País: | United Kingdom
Título: | A novel strategy for the targeted analysis of protein and peptide metabolites
Autores: | Nicholas A. Williamson; Charles Reilly; Chor-Teck Tan; Sri-Harsha Ramarathinam; Alun Jones; Christie L. Hunter; Francis R. Rooney; Anthony W. Purcell.
Data: | 2009-09-29
Ano: | 2009
Palavras-chave: | Biotechnology; Immunology; Pharmacology.
Resumo: | The detection and quantitation of exogenously administered biological macromolecules (e.g. vaccines, peptide and protein therapeutics) and their metabolites is frequently complicated by the presence of a complex endogenous mixture of closely related compounds. We describe a method that incorporates stable isotope labeling of the compound of interest allowing the selective screening of the intact molecule and all metabolites using a modified precursor ion scan. This method involves monitoring the low molecular weight fragment ions produced during MS/MS that distinguish isotopically labelled material from related endogenous compounds. All isotopically labelled substances can be selected using this scanning technique for further analysis whilst other unlabelled and irrelevant substances are ignored. The potential for this technique to be used in metabolism and pharmacokinetic experiments is discussed with specific examples looking at the metabolism of &amp;#x3b1;-synuclein in serum and the brain.
Tipo: | Manuscript

Identificador:

http://precedings.nature.com/documents/3812/version/1

oai:nature.com:10101/npre.2009.3812.1

http://hdl.handle.net/10101/npre.2009.3812.1

Fonte: | Nature Precedings
Fonte: | Creative Commons Attribution 3.0 License