Data/hora: 05/06/2024 18:23:47

Provedor de dados: 	17	
País: 	United Kingdom	
Título: 	Clonal origin of Epstein-Barr virus-infected T/NK-cell subpopulations in chronic active Epstein-Barr virus infection	
Autores: 	Shouichi Ohga; Masataka Ishimura; Goichi Toshimoto; Toshihiro Miyamoto; Hidetoshi Takada; Tamami Tanaka; Koichi  Ohshima; Ken-Ichi Imadome; Yasunobu Abe; Koichi Akashi; Toshiro Hara.	
Data: 	2010-02-25	
Ano: 	2010	
Palavras-chave: 	Immunology; Microbiology.	
Resumo: 	Clonal expansion of Epstein-Barr virus (EBV) infected B-cells occasionally occurs in immunocompromized subjects. EBV-infected T/natural killer (NK)-cells proliferate in patients with chronic active EBV infection (CAEBV) that is a rare mononucleosis syndrome. It is classified into either T-cell type or NK-cell type according to the primary target of infection, while the pathogenesis remains unclear. To search the clonal origin of EBV-infected T/NK-cells, virus distribution and clonotype were assessed by using highly purified cell fractions obtained from 6 patients. Patient 1 had a monoclonal proliferation of EBV-infected T-cell receptor V&amp;#x3b4;2/V&amp;#x3b3;9-expressing cells, and carried lower copy number of EBV in &amp;#x3b1;&amp;#x3b2;T-cells. Patients 2 and 3 had a clonal expansion of EBV-infected CD4+T-cells, and lower EBV load in CD56+cells. Patients 4, 5 and 6 had an expansion of CD56+cells with higher EBV load than CD3+cells. EBV-terminal repeats were determined as clonal bands in the minor targeted populations of 5 patients. The size of terminal repeats indicated the same clonotype in minor subsets as in major subsets of 4 patients. However, EBV was not detected in bone marrow-derived lineage negative CD34+cells of patients. These results suggested that EBV could infect T/NK-cells at differentiation stage, but spared bone marrow CD34+hematopoietic stem cells in CAEBV patients.	
Tipo: 	Manuscript	
Identificador: 	http://precedings.nature.com/documents/4238/version/1

oai:nature.com:10101/npre.2010.4238.1

http://hdl.handle.net/10101/npre.2010.4238.1	
Fonte: 	Nature Precedings	
Fonte: 	Creative Commons Attribution 3.0 License