Data/hora: 01/06/2024 06:48:02

Provedor de dados: | 190
País: | Brazil

Título:

Methodology for sporulation and production of monoconidial isolates of Sphaeropsis sapinea

Metodologia para esporulação e produção de culturas monospóricas de Sphaeropsis sapinea

Autores: | Basilio, Paula Rachel Rabelo Corrêa; Auer, Celso Garcia; Santos, Álvaro Figueredo dos; Higa, Antonio Rioyei.
Data: | 2010-06-28
Ano: | 2010
Palavras-chave: | Inoculum; Pathogen; Pine. Inóculo; Patógeno; Pínus.
Resumo: | Sphaeropsis sapinea is one of main pathogens that causes losses in commercial plantations of Pinusspp. Studies for control based on genetic resistance can be done by inoculating different monosporic isolates onpine populations and selectioning the resistant individuals. This work describes a methodology for pycnidia production in vitro and S. sapinea monoconidial cultures, using an agar-water medium with sterile pine needles. Four S. sapinea isolates were tested e pycnidia and mature conidia were observed after 14 days of incubation, under continuous fluorescent light, in BOD, at 25 °C. Pycnidia were triturated in sterile water and conidia germinated after two hours of incubation in water-agar, in BOD, at 25 °C. Germinated conidia formed colonies which were purified in malt extract-agar and maintained in test tubes with the same medium, covered with sterile mineral oil for preservation. This methodology revealed to be  adequated for production of monoconidial coloniesof S. sapinea
Tipo: | Info:eu-repo/semantics/article
Idioma: | Português
Identificador: | http://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/139
Editor: | Embrapa Florestas
Relação: | http://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/139/99
Formato: | application/pdf

Fonte:

Brazilian Journal of Forestry Research; n. 54 (2007): jan./jun. Pesquisa Florestal Brasileira; 145

Pesquisa Florestal Brasileira; n. 54 (2007): jan./jun. Pesquisa Florestal Brasileira; 145

1983-2605

1809-3647

Fonte:

Direitos autorais 2015 Pesquisa Florestal Brasileira

http://creativecommons.org/licenses/by-nc-nd/4.0