Data/hora: 02/06/2024 05:01:07

Provedor de dados: | 190
País: | Brazil

Título:

Isolamento de DNA genômico de indivíduo de nematoide parasita de insetos: estudo de caso de Deladenus

Individual genomic DNA isolation of Entomogenous nematodes: the Deladenus study case

Autores: | Schuhli, Guilherme Schnell e; Penteado, Susete do Rocio Chiarello; Kestring, Daiane Rigoni; Steil, Gisleine Jarenko; Thomas, Micheli Cristina.
Data: | 2012-12-17
Ano: | 2012
Palavras-chave: | Genética Molecular; Entomologia Florestal; Controle Biológico DNA extraction; Protocol; Woodwasp; Sirex Noctilio Extração de DNA; Protocolo; Vespa-da-madeira; Sirex Noctilio.
Resumo: | Populational studies involve single individual DNA extraction in order to grant further genotyping data. In the case of some parasitic nematodes, the reduced dimensions and high individual number per infestation makes individual genotyping a difficult task. Aiming the development of a protocol we performed adjustments in available methods in order to acquire the best gain in purity and concentration of genomic DNA. Single specimens were digested in Worm Lysis Buffer and submitted to PCR amplification as a concept test. It was possible to obtain good amount and concentration of DNA from individuals. Quality was sufficient to grant subsequent ITS1 sequencing.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês

Identificador:

http://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/396

10.4336/2012.pfb.32.72.483

Editor: | Embrapa Florestas

Relação:

http://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/396/293

http://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/downloadSuppFile/396/286

http://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/downloadSuppFile/396/287

Formato: | application/pdf

Fonte:

Pesquisa Florestal Brasileira; v. 32, n. 72 (2012); 483

Brazilian Journal of Forestry Research; v. 32, n. 72 (2012); 483

1983-2605

1809-3647

Fonte:

Direitos autorais 2015 Pesquisa Florestal Brasileira

http://creativecommons.org/licenses/by-nc-nd/4.0