Data/hora: 11/06/2024 08:03:44

Provedor de dados: | 19
País: | Japan

Título:

Freeze-drying Spermatozoa in Mice

凍結乾燥によるマウス精子の保存

Autores: | 鈴木, 宏志; 川瀬, 洋介.
Data: | 2008
Ano: | 2008
Palavras-chave: | Mice; Freese-drying; Spermatozoa; マウス; 凍結乾燥; 精子.
Resumo: | Cryopreservation of mouse spermatozoa has been widely applied for maintenance of genetically modified mouse strains. Although cryopreservation of mouse spermatozoa is simpler, less time-consuming, and less costly than of embryos for preservation of the genetic materials, maintenance of cryopreserved spermatozoa still has a high running cost because of the need for a constant supply of liquid nitrogen. In 1998, it has been reported that freeze-dried mouse spermatozoa are capable of participating in normal embryonic development after injection into oocytes, and so they have attracted a great deal of attention as storable gene resources. However, attempts of freeze-dry spermatozoa are not new. Successful conception and full-term development using freeze-dried spermatozoa were reported in the cow and rabbit prior to 1960. Unfortunately, these preliminary results have not been confirmed. Spermatozoa become defective in motility after freeze-drying and are unable to fertilize eggs both in vivo and in vitro. Since freeze-drying of mammalian spermatozoa was demonstrated without loss of genetic or reproductive potential, recent investigations of freeze-dried sperm have focused on the factors affecting freeze-drying spermatozoa in an effort to store the male gamete at ambient temperature. It is particularly essential to assure long-term preservation for several decades or centuries. Recent findings indicate that the pressure levels at primary drying of freeze-dried spermatozoa, in addition to the components of the suspending solution, appears to be an important factor for long-term preservation of freeze-dried spermatozoa in mice.
Idioma: | Japonês

Identificador:

http://ir.obihiro.ac.jp/dspace/handle/10322/4510

info:doi/10.20585/cryobolcryotechnol.54.1_21

Editor: | 低温生物工学会
Fonte: | 低温生物工学会