Data/hora: 02/06/2024 15:56:35

Provedor de dados: | 19
País: | Japan
Título: | Gene and protein expression of a soluble form of CTLA-4 in a healthy dog
Autores: | Tagawa, Michihito; Yamamoto, Yuhei; Shimbo, Genya; Iguchi, Aiko; Xuan, Xuenan; Tomihari, Mizuki; Miyahara, Kazuro.
Data: | 2017-05
Ano: | 2017
Palavras-chave: | CD152; CTLA-4; Dog; Soluble form; Splicing variant.
Resumo: | Cytotoxic T lymphocyte associated gene-4 (CTLA-4) is a costimulatory molecule, expressed on the surface of activated T cells that negatively regulates T cell activation. In humans, alternative splicing of the CTLA-4 gene generates two major isoforms of mRNA, and a soluble form of CTLA-4 (sCTLA-4) was detected in normal human serum. We describe alternatively spliced mRNA expressed in peripheral blood mononuclear cells obtained from a healthy dog lacking the transmembrane domain coded by exon 3 of the CTLA-4 gene. Immunoprecipitation and western blotting of dog serum revealed a band of approximately 23-kDa, which is consistent with the predicted size, based on the amino acid sequence of the canine sCTLA-4 obtained in this study.
Idioma: | Inglês

Identificador:

http://ir.obihiro.ac.jp/dspace/handle/10322/4517

info:doi/10.1292/jvms.16-0583

Editor: | Japanese Society of Veterinary Science
Fonte: | This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-ncnd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)