Data/hora: 05/06/2024 01:19:53

Provedor de dados: | 19
País: | Japan
Título: | Detection of monoclonal integration of bovine leukemia virus proviral DNA as a malignant marker in two enzootic bovine leukosis cases with difficult clinical diagnosis
Autores: | MIURA, Saori; HORIUCHI, Noriyuki; MATSUMOTO, Kotaro; KOBAYASHI, Yoshiyasu; KAWAZU, Shin-ichiro; INOKUMA, Hisashi.
Data: | 2015
Ano: | 2015
Palavras-chave: | Atypical enzootic bovine leukosis; Diagnostic marker; Monoclonal integration.
Resumo: | Monoclonal integration of bovine leukemia virus (BLV) proviral DNA into bovine genomes was detected in peripheral blood from two clinical cases of enzootic bovine leukosis (EBL) without enlargement of superficial lymph nodes. A BLV-specific probe hybridized with 1 to 3 EcoRI and HindIII fragments in these 2 atypical EBL cattle by Southern blotting and hybridization, as well as in 3 typical EBL cattle. The probe also hybridized to a large number of EcoRI and HindIII fragments in 5 cattle with persistent leukosis. These results suggest that the detection of monoclonal integration of BLV provirus into the host genome may serve as a marker of monoclonal proliferation and malignancy in difficult to diagnose EBL cattle.
Idioma: | Inglês
Identificador: | http://ir.obihiro.ac.jp/dspace/handle/10322/4143
Editor: | Japanese Society of Veterinary Science
Fonte: | This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License &lt;http://creativecommons.org/licenses/by-nc-nd/3.0/&gt;.