Data/hora: 02/06/2024 11:51:03

Provedor de dados: | 19
País: | Japan

Título:

ウシ優性卵胞と閉鎖卵胞の卵胞液中におけるN-アセチルグルコサミニルトランスフェラーゼ I 活性の解析

N-Acetylglucosaminyltransferase I Activity in Bovine Ovarian Follicular Fluids from Dominant and Atretic Follicles

Autores: | Nozaki, Hirofumi; Miyamoto, Akio; Hayashi, Ken-Go; Matsui, Motozumi; Yoshida, Takashi; Nakamura, Tadashi; Arai, Ikichi; Urashima, Tadasu; 宮本, 明夫; 松井, 基純; 中村, 正; 浦島, 匡.
Data: | 2004
Ano: | 2004
Palavras-chave: | N-acetylglucosaminyltransferase I; Dominant follicle; Atretic follicle; Follicular fluid; Bovine.
Resumo: | The activity of α3-D-mannoside-β-1,2-N-acetylglucosaminyltransferase I (GnT I; EC 2.4.1.101), which catalyzes the first step in the conversion of oligomannose to complex or hybrid N-glycans of glycoproteins, was detected in bovine follicular fluid (bFF). The GnT I activity in bFF had a pH optimum of 5.8 and an absolute requirement for either Co2+, Mn2+, or Mg2+, the activity being stimulated by these cations in the above order. The apparent Km value for α1-3α1-6 mannopentaose of GnT I in bFF was 2.17 mM. The substrate specificity for the GnT I activity decreased in the following order: α1-3α1-6 mannopentaose&gt; α1-3α1-6 mannotriose&gt;α1-3 mannobiose. The GnT I activity in bFF from large atretic follicles was significantly higher than in that from large dominant follicles. Moreover there was no significant difference between the GnT I activities in bFF from dominant follicles collected before and after surge of luteinizing hormone (LH surge). These data suggest that the GnT I activity in bFF may reflect functional changes in the microenvironment which lead to follicular atresia.
Idioma: | Inglês
Identificador: | http://ir.obihiro.ac.jp/dspace/handle/10322/4185
Editor: | 日本応用糖質科学会
Fonte: | 日本応用糖質科学会