Data/hora: 11/06/2024 11:08:20

Provedor de dados: | 19
País: | Japan
Título: | Construction of the recombinant pseudorabies viruses expressing Cryptosporidium parvum an immunodominant surface protein, p23
Autores: | Takashima, Yasuhiro; Xuan, Xuenan; Nagasawa, Hideyuki; Matsumoto, Yasunobu; Igarashi, Ikuo; Fujisaki, Kozo; Mikami, Takeshi; Otsuka, Haruki; 玄, 学南; 五十嵐, 郁男.
Data: | 2000-10
Ano: | 2000
Palavras-chave: | Cryptosporidium parvum; P23; Herpes; Pseudorabies virus; Subunit vaccine.
Resumo: | To develop a vaccine against cryptosporidiosis in animals, we constructed recombinant pseudorabies virus (PrV), a member of the Herpesviridae Alphaherpesvirus subfamily, expressing an immunodominant surface protein p23 of Cryptosporidium parvum sporozoites. Because of the wide host range of PrV, it has the possibility as the vector to delivery the foreign genes to several species of animals containing experiment animal. In the recombinant constructed in this study, the p23 gene under the control of CAG promoter was integrated into the thymidine kinase (TK) gene of PRV. Antibody against p23 recognized p23 expressed in CPK cells infected with the recombinant, as the approximate 23 kDa specific band in Western blotting analysis. This study showed the possibility of a PrV recombinant as a vaccine against cryptosporidiosis in animals.
Idioma: | Inglês
Identificador: | http://ir.obihiro.ac.jp/dspace/handle/10322/129
Editor: | National Research Center for Protozoan Diseases
Formato: | application/pdf
Fonte: | National Research Center for Protozoan Diseases