Data/hora: 01/06/2024 01:23:17

Provedor de dados: | 19
País: | Japan
Título: | Intraluteal Release of Prostaglandin F2α and E2 During Corpora Lutea Development in the Cow
Autores: | KOBAYASHI, Shu-ichi; ACOSTA, Tomas J.; HAYASHI, Kanako; BERISHA, Bajram; OZAWA, Takahiro; OHTANI, Masayuki; SCHAMS, Dieter; MIYAMOTO, Akio; 宮本, 明夫.
Data: | 2002
Ano: | 2002
Palavras-chave: | PGF2α; PGE2; Developing CL; Cow; In vivo.
Resumo: | It is well known that prostaglandin (PG) F2α and PGE2 are actively produced by bovine early corpus luteum (CL) at higher levels than in the CL of later stages. However, there is no in vivo information about the local secretion of PGs within bovine developing CL so far. Thus, the objective of the present study was to determine in detail the real-time changes in PGF2α and PGE2 secretion within developing CL. A microdialysis system (MDS) was surgically implanted into the newly formed CL of 6 cows on Day 3 after GnRH administration (about 1.5 days after ovulation) to induce superovulation following FSH treatment. Both PGF2α and PGE2 release were high during the first 24 h within developing CL but decreased thereafter. These profiles were well reflected in the PG concentrations in ovarian venous plasma. Furthermore, reverse transcription polymerase chain reaction analysis using CL from other animals independent from the MDS study revealed that the level of cyclo-oxyganase-2 mRNA expression in early CL was higher than that of later luteal phases. The present results provide the first direct in vivo evidence that active PGs production in CL occurs up to Day 4 after estrus in the cow.
Idioma: | Inglês
Identificador: | http://ir.obihiro.ac.jp/dspace/handle/10322/2974
Editor: | 日本繁殖生物学会
Formato: | application/pdf
Fonte: | 日本繁殖生物学会