Data/hora: 11/06/2024 02:30:49

Provedor de dados: 	19	
País: 	Japan	
Título: 	Toxoplasma gondii: DNA vaccination with genes encoding antigens MIC2, M2AP, AMA1 and BAG1 and evaluation of their immunogenic potential	
Autores: 	Dautu, George; Munyaka, Biscah; Carmen, Gabriella; Zhang, Guohong; Omata, Yoshitaka; Xuan, Xuenan; Igarashi, Makoto.	
Data: 	2007-07	
Ano: 	2007	
Palavras-chave: 	Toxoplasma gondii; DNA; Deoxyribonucleic acid; DNA immunization; MIC2; Microneme protein-2; M2AP; MIC2 associated protein; AMA1; Apical membrane antigen-1; BAG1; Bradyzoite antigen-1; IgG; Immunoglobulin G.	
Resumo: 	A combination of antigenic regions of microneme proteins have been previously reported as being protective against chronic toxoplasmosis. In this work, we evaluated immune responses induced by immunizing BALB/c and C57BL/6 mice intradermally with plasmid DNA encoding the protein sequences of Toxoplasma gondii AMA1, MIC2, M2AP and BAG1. Mice immunized with the AMA1 gene developed high levels of serum IgG2a and c antibodies as well as cellular immune responses associated with IFN-γ synthesis suggesting a modulated Th1 type of response. Immunization with the AMA1 gene resulted in a partial but significant protection against the acute phase of toxoplasmosis compared to MIC2, M2AP and BAG1 genes. Therefore, the AMA1 gene appears to generate a strong specific immune response and also provides effective protection against toxoplasmosis more than the MIC2, M2AP and BAG1 genes.	
Idioma: 	Inglês	
Identificador: 	http://ir.obihiro.ac.jp/dspace/handle/10322/1031	
Editor: 	Elsevier	
Formato: 	application/pdf