Data/hora: 11/06/2024 05:21:53

Provedor de dados: | 19
País: | Japan
Título: | DNA vaccine coding a p23 protein of Cryptosporidium parvum fused with Fc portion of immunogloblin G induces higher level of interferon-γ expression in mice
Autores: | Takashima, Yasuhiro; Xuan, Xuenan; Shirafuji, Hiroaki; Zhang, Guohong; Otsuka, Haruki.
Data: | 2005
Ano: | 2005
Palavras-chave: | C. parvum; DNA vaccine; Fc; Interferon-8.
Resumo: | To develop DNA vaccines against Cryptosporidiosis, a plasmid coding an immunodominant protein of Cryptosporidium parvum sporozoite, p23 (pCX-p23) and another plasmid coding a fusion protein containing whole the p23 and the Fc portion of mouse immunogloblin G1 (pCX-p23Fc). Vaccination of BALB/c mice with the plasmid, pCX-p23 and pCX-p23Fc induced the production of antibodies against p23. Although both of splenocytes of mice immunized with the plasmids pCX-p23 and pCX-p23Fc expressed interleukin-4 and interferon-8, after the in vitro stimulation by p23 antigen, the interferon-8 expression level of pCX-p23Fc immunized mice was much higher than that of pCX-p23 immunized mice. These results suggest a possibility  of the plasmid pCX-p23Fc as a DNA vaccine candidate against cryptosporidiosis.
Idioma: | Inglês
Identificador: | http://ir.obihiro.ac.jp/dspace/handle/10322/147
Editor: | National Research Center for Protozoan Deseases
Formato: | application/pdf