Data/hora: 05/06/2024 13:51:51

Provedor de dados: 	19	
País: 	Japan	
Título: 	Relative Changes in mRNA Expression of Angiopoietins and Receptors Tie in Bovine Corpus Luteum during Estrous Cycle and Prostaglandin F2α-induced Luteolysis: A Possible Mechanism for the Initiation of Luteal Regression	
Autores: 	TANAKA, Jun; ACOSTA, Tomas J.; BERISHA, Bajram; TETSUKA, Masa; MATSUI, Motozumi; KOBAYASHI, Shuichi; SCHAMS, Dieter; MIYAMOTO, Akio; 松井, 基純; 宮本, 明夫.	
Data: 	2004	
Ano: 	2004	
Palavras-chave: 	Angiopoietin; Angiolysis; Luteolysis; Corpus luteum; Cow.	
Resumo: 	Local angiogenesis and angiolysis in the corpus luteum (CL) relate to the luteal function. Recent studies indicate that angiopoietins (ANPT) and their receptors Tie regulate remodeling of microvasculature. We therefore examined 1) the relative changes in the expression of mRNA for ANPT-1, ANPT-2, Tie1 and Tie2 in bovine CL by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) during the estrous cycle and prostaglandin F2α (PGF2α)-induced luteolysis, and 2) the effect of ANPT-2 on progesterone (P4) release from CL at the late stage of the estrous cycle by an in vitro microdialysis system (MDS). The CLs were classified into 4 stages (early: Day 2-5, n=7, mid: Day 8-12, n=15, late: Day 15-17, n=9, regressing: Day &gt;18, n=19). The levels of ANPT-1 mRNA in early and regressing CL were lower than those in mid and late CL, whereas ANPT-2 mRNA expression did not change during the estrous cycle. The Tie2 mRNA expression decreased as the CL aged. During PGF2α-induced luteolysis, ANPT-2 mRNA expression was acutely and temporally increased at 2 h after PGF2α injection. The expression of ANPT-1 mRNA was decreased from 4 h after PGF2α injection and kept low levels. In the experiment with the in vitro MDS, an infusion of ANPT-2 (100 ng/ml) acutely inhibited P4 release from late CL. Overall, results suggest that decrease of ANPT-1 mRNA is a basic mechanism of vascular remodeling in CL. In addition, ANPT-2 might play a role in regulation of P4 secretion in CL during luteolysis.	
Idioma: 	Inglês	
Identificador: 	http://ir.obihiro.ac.jp/dspace/handle/10322/2977	
Editor: 	日本繁殖生物学会	
Formato: 	application/pdf	
Fonte: 	日本繁殖生物学会