Data/hora: 05/06/2024 08:22:53

Provedor de dados: | 19
País: | Japan
Título: | Integrin-linked kinase is involved in lactoferrin-induced anchorage-independent cell growth and survival in PC12 cells
Autores: | Ishii, Toshiaki; Uto, Tomoka; Mori, Kaori; Fujikawa, Ryu; 石井, 利明.
Data: | 2009-04-10
Ano: | 2009
Palavras-chave: | Bovine lactoferrin; PC12 cell; Integrin-linked kinase; Integrin; P38 MAPK; Cell suspension; Cell adhesion; Cell growth; Cell viability; Dominant-negative mutant; Anchorage-independent growth.
Resumo: | Aims: Bovine lactoferrin (bLf) causes anchorage-independent cell growth in PC12 cells. The present study investigated the mechanisms involved in bLf-induced anchorage-independent cell growth and survival in PC12 cells. Main methods: The number of adherent cells and suspended cells was estimated separately by using a methyl thiazol tetrazolium (MTT) assay, and the sum of both optical density (O.D.) (570 nm) values was used as a measure of the total number of cells. Key findings: Integrin-linked kinase (ILK) plays an important role in integrin and growth factor signaling pathways. Stable transfection of PC12 cells with a dominant negative kinase-deficient mutant of ILK (DN-ILK) inhibited bLf-induced anchorage-independent cell growth. The ILK activity in the parental cells was transiently activated after addition of bLf, whereas bLf-induced activation of ILK was blocked in DN-ILK-transfected cells. bLf also activated p38 mitogen-activated protein kinase (MAPK); however, the p38 MAPK activation was inhibited by stable DN-ILK transfection. Moreover, cell viability in the suspended cells by bLf strongly decreased after treatment with SB203580, an inhibitor of p38 MAPK. Significance: These results suggest that ILK is involved in bLf-induced anchorage-independent cell growth and viability via activation of p38 MAPK.
Idioma: | Inglês
Identificador: | http://ir.obihiro.ac.jp/dspace/handle/10322/2675
Editor: | Elsevier
Formato: | application/pdf
Fonte: | Elsevier