Data/hora: 10/06/2024 18:15:47

Provedor de dados: | 19
País: | Japan

Título:

コモンウオンバットミルクオリゴ糖の構造解析

Chemical characterization of milk oligosaccharides of the common wombat (Vombatus ursinus)

Autores: | Hirayama, Kentaro; Taufik, Epi; Kikuchi, Megumi; Nakamura, Tadashi; Fukuda, Kenji; Saito, Tadao; Newgrain, Keith; Green, Brian; Messer, Michael; Urashima, Tadasu.
Data: | 2016-09
Ano: | 2016
Palavras-chave: | Common wombat; Marsupial; Milk oligosaccharide; Vombatus ursinus.
Resumo: | Previous structural characterizations of marsupial milk oligosaccharides have been performed in the tammar wallaby, red kangaroo, koala, common brushtail possum and the eastern quoll. To clarify the homology and heterogeneity of milk oligosaccharides among marsupial species, which could provide information on their evolution, the oligosaccharides of wombat milk carbohydrate were characterized in this study. Neutral and acidic oligosaccharides were isolated from the carbohydrate fractions of two samples of milk of the common wombat and characterized by H-1-nuclear magnetic resonance spectroscopy. The structures of six neutral saccharides were found to be Gal(1-4)Glc (lactose), Gal(1-3)Gal(1-4)Glc (3'-galactosyllactose), Gal(1-3)Gal(1-3)Gal(1-4)Glc (3',3-digalactosyllactose), Gal(1-3)Gal(1-3)Gal(1-3)Gal(1-4)Glc, Gal(1-3)Gal(1-3)[Gal(1-4)GlcNAc(1-6)]Gal(1-4)Glc (galactosyl lacto-N-novopentaose I) and Gal(1-3)[Gal(1-4)GlcNAc(1-6)]Gal(1-3)[Gal(1-4)GlcNAc(1-6)]Gal(1-4)Glc (lacto-N-novooctaose), while those of six acidic saccharides were Neu5Ac(2-3)Gal(1-3)Gal(1-4)Glc. (sialyl 3'-galactosyllactose), Neu5Ac(2-3)Gal(1-3)Gal(1-3)Gal(1-4)Glc (sialyl 3',3-digalactosyllactose), Neu5Ac(2-3)Gal(1-3)[Gal(1-4)GlcNAc(1-6)]Gal(1-4)Glc (sialyl lacto-N-novopentaose a), Gal(1-3)[Neu5Ac(2-3)Gal(1-4)GlcNAc(1-6)]Gal(1-4)Glc (sialyl lacto-N-novopentaose c), Neu5Ac(2-3)Gal(1-3)Gal(1-3)Gal(1-3)Gal(1-4)Glc,, Neu5Ac(2-3)Gal(1-3)Gal(1-3)[Gal(1-4)GlcNAc(1-6)]Gal(1-4)Glc and Gal(1-3)Gal(1-3)[Neu5Ac(2-3)Gal(1-4)GlcNAc(1-6)]Gal(1-4)Glc. In addition, small amounts of sulfated oligosaccharides but no oligosaccharides containing Neu5Gc or (2-6) linked Neu5Ac were detected.
Idioma: | Inglês

Identificador:

http://ir.obihiro.ac.jp/dspace/handle/10322/4471

info:doi/10.1111/asj.12566

Editor: | Wiley-Blackwell
Fonte: | This is the peer reviewed version of the following article: Animal Science Journal: 87(9); SEP 2016; 1167-1177, which has been published in final form at http://doi.org/10.1111/asj.12566. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.