Data/hora: 02/06/2024 03:58:31

Provedor de dados: 	19	
País: 	Japan	
Título: 	Upregulation of interferon-stimulated genes and interleukin-10 in peripheral blood immune cells during early pregnancy in dairy cows.	
Autores: 	MAGATA, Fumie; SHIRASUNA, Koumei; STRÜVE, Klaas; HERZOG, Kathrin; SHIMIZU, Takashi; BOLLWEIN, Heinrich; MIYAMOTO, Akio; 清水, 隆; 宮本, 明夫.	
Data: 	2012	
Ano: 	2012	
Palavras-chave: 	Dairy cow; Early pregnancy; Persistent corpus luteum; MRNA expression.	
Resumo: 	Persistence of the corpus luteum (CL) in cattle usually occurs during the puerperium and is associated with interference of prostaglandin (PG) F2α release from the uterus. The objective of the present study was to determine for the first time the gene expressions in the persistent CL compared with the CL of pregnancy and cyclic CL. Three types of CL biopsy samples were collected from 32 lactating Holstein cows: (1) CL persisting for 29 to 33 days after the first ovulation postpartum (persistent CL, n=9), (2) CL between days 29 and 33 of early pregnancy (CL of pregnancy, n=8) and (3) CL between days 10 and 13 of the estrous cycle (cyclic CL, n=27). mRNA expression of 2',5'-oligoadenylate synthetase-1 was upregulated only in the CL of pregnancy, confirming exposure to interferon-τ (IFNT) produced by trophoblasts in pregnant cows. mRNA expressions of immune tolerance-related factors (PGES and forkhead/winged helix transcription factor 3) were upregulated in the CL of pregnancy but not in the persistent CL, suggesting that IFNT controls upregulation of these genes. mRNA expression relating to some of the major systems such as lymphangiogenesis, inflammation and apoptosis were similarly upregulated in the persistent CL and the CL of pregnancy but not in the cyclic CL. The results suggest that the persistent CL may survive for a long period without changes in local immune tolerance but develops several major systems required for CL maintenance similar to the CL of pregnancy.	
Idioma: 	Inglês	
Identificador: 	http://ir.obihiro.ac.jp/dspace/handle/10322/4039	
Editor: 	日本繁殖生物学会	
Fonte: 	日本繁殖生物学会