Data/hora: 13/06/2024 16:11:27

Provedor de dados: 	19	
País: 	Japan	
Título: 	Effects of Euglena (Euglena gracilis) supplemented to diet (forage: concentrate ratios of 60:40) on the basic ruminal fermentation and methane emissions in in vitro condition	
Autores: 	Aemiro, Ashagrie; Watanabe, Shota; Suzuki, Kengo; Hanada, Masaaki; Umetsu, Kazutaka.	
Data: 	2016-02	
Ano: 	2016	
Palavras-chave: 	Methane emission; Euglena; Protozoa; Volatile fatty acid; Digestibility; Ammonia.	
Resumo: 	An in vitro study was conducted to investigate the effect of different concentrations of Euglena (Euglena gracilis) on CH4 production, dry matter (DM) digestibility, volatile fatty acid (VFA) and ammonia N(NH3-N) concentration as well as on the protozoa population. The treatments considered were Euglena at concentrations of 0.0, 50, 100, 200, 400 and 1000 g/kg dry matter (DM) of the substrate (60:40 forage: concentrate ratio) incubated for 24 and 96 h using an in vitro continuous gas production and in vitro two-stage digestion procedure, respectively. The data were subjected to polynomial regression analysis. Methane emissions (ml/g DM) decreased at an increasing rate, generally with increasing concentration of Euglena but also exhibited quadratic (P&lt; 0.001) and cubic (P&lt; 0.001) effects while NH3-N (mg/ml) concentration increased at an increasing rate (linear P &lt; 0.001; quadratic P= 0.001; cubic P= 0.024). Total VFA concentration (mmol/l) decreased significantly (P&lt; 0.001), when the substrate was totally replaced by Euglena. There was a linear (P&lt; 0.001) and cubic (P= 0.047) reduction in protozoa population as the concentration of Euglena increased. In vitro DM digestibility was improved (linear P= 0.003; quadratic P=0.04; cubic P&lt;0.001). These findings demonstrate that Euglena at concentration of 100 g/kg DM reduce CH4 emissions by 9.1% and improve DM digestibility by 14.3%. However, when the concentration of Euglena increases from 100 g/kg DM, while further reducing CH4 emissions, have negative effect on NH3-N concentration, protozoa population and VFA concentration.	
Idioma: 	Inglês	
Identificador: 	http://ir.obihiro.ac.jp/dspace/handle/10322/4458

info:doi/10.1016/j.anifeedsci.2015.12.010	
Editor: 	Elsevier	
Fonte: 	This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).