Data/hora: 07/06/2024 09:07:39

Provedor de dados: 	22	
País: 	Brazil	
Título: 	Gel filtration chromatography for the purification of protein and glucosidic molecules from phytopathogenic nematodes

Cromatografia de filtração em gel para a purificação de moléculas protéicas e glicídidas a partir de nematóides fitopatogênicos	
Autores: 	Gonçalves, Edilaine Della Valentina; Stangarlin, José Renato; Roncato, Sidiane Coltro; Dildey, Omari Dangelo Forlin; Meinerz, Cristiane Cláudia; Kuhn, Odair José.	
Data: 	2014-12-14	
Ano: 	2014	
Palavras-chave: 	Meloidogyne incognita; Meloidogyne javanica; Eliciadores.	
Resumo: 	- Female root-knot nematode can present compounds able to activate defense mechanisms in the vegetable. With this end, the chromatographic technique is based on the purification of molecules such as proteins, glycoproteins, nucleic acids and polysaccharides, which may present eliciting characteristics. The aim of this paper was to determine the elicitors purification methodology, from phytopathogenic nematodes, by gel filtration chromatography (GFC). Nematodes females of Meloidogyne incognita and Meloidogyne javanica were extracted, macerated, and subsequently subjected to gel filtration chromatography. For the M. incognita five protein peaks and seven glucosidic peaks, with molecular masses ranging from 8.80 to 285.95 KDa and from 65.09 to 7.47 µg glucose mL-1, respectively, were obtained. For the M. javanica it was obtained four protein peaks, which masses ranged from 304.99 to 108.73 kDa. By GFC was possible to purify protein and glucosidic fractions from female M. incognita and M. javanica that will be used later for resistance induction assays in plants.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Português	
Identificador: 	http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/11175

10.1818/sap.v13i0.11175	
Editor: 	Scientia Agraria Paranaensis	
Relação: 	http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/11175/8002	
Formato: 	application/pdf	
Fonte: 	Scientia Agraria Paranaensis; Vol. 13 - Suplemento (2014); 353-357

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