Data/hora: 06/06/2024 05:51:35

Provedor de dados: 	27	
País: 	Belgium	
Título: 	Uptake of &lt;i&gt;Vibrio anguillarum&lt;/i&gt; vaccine by &lt;i&gt;Artemia salina&lt;/i&gt; as a potential oral delivery system to fish fry	
Autores: 	Campbell, R.; Adams, A.; Tatner, M.F.; Chair, M.; Sorgeloos, P.	
Data: 	1993	
Ano: 	1993	
Palavras-chave: 	Artemia salina; Vibrio anguillarum.	
Resumo: 	An ELISA to detect &lt;i&gt;Vibrio anguillarum&lt;/i&gt; antigen in &lt;i&gt;Artemia salina&lt;/i&gt; was developed. This assay was then used to determine the time course of antigen uptake by &lt;i&gt;Artemia&lt;/i&gt; during incubations with &lt;i&gt;Vibrio&lt;/i&gt; vaccine with a view to using &lt;i&gt;Artemia&lt;/i&gt; as a vaccine carrier for oral vaccination of fish fry. The rate of uptake of vaccine by &lt;i&gt;Artemia&lt;/i&gt; was shown to be concentration dependent. At a dose of 1.5x10&lt;sup&gt;7&lt;/sup&gt; cfu ml&lt;sup&gt;-1&lt;/sup&gt;, 1 mg wet weight of &lt;i&gt;Artemia&lt;/i&gt; took up 1x10&lt;sup&gt;5&lt;/sup&gt; cfu within 30min and reached maximum of 6x10&lt;sup&gt;6&lt;/sup&gt; cfu after 60min. Thus each &lt;i&gt;Artemia&lt;/i&gt; appears to have taken up approximately 1.2x10&lt;sup&gt;5&lt;/sup&gt; cfu. Comparison of vaccine formulations indicated that uptake was consistently higher with formalised than with heat-treated vaccine.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2668	
Fonte: 	%3Ci%3EFish+Shellfish+Immunol.+3%286%29%3C%2Fi%3E%3A+451-459	
Fonte: 	info:eu-repo/semantics/restrictedAccess