Data/hora: 02/06/2024 12:34:24

Provedor de dados: | 27
País: | Belgium
Título: | Sequential determination of a combined gamma/ß and pure ß-emitter by gamma and liquid scintillation counting: application to the transport of metals across fish gills
Autores: | Van Ginneken, L.; Blust, R.
Data: | 1995
Ano: | 1995
Resumo: | A combined gamma scintillation/liquid scintillation technique for the sequential determination of two radioactive tracers is described. The method was developed using a combined gamma/ß-emitter (&lt;sup&gt;57&lt;/sup&gt;Co) and a pure ß-emitter (&lt;sup&gt;45&lt;/sup&gt;Ca). First, the &lt;sup&gt;57&lt;/sup&gt;Co radioactivity was determined by counting the samples in a gamma scintillation analyzer in the energy region 80-165 keV. Next, the samples were counted in a liquid scintillation analyzer. Only one energy region was used to count both isotopes to maximize the counting efficiencies. From the difference between quenched and unquenched ß-spectra, the counting region was set from 0 to 256 keV. The counting efficiency was related to a quench-indicating parameter (tSIE) for both nuclides by fitting a rectangular hyperbola to the quench data. By subtracting the &lt;sup&gt;57&lt;/sup&gt;Co counts from the observed counts in the total window, &lt;sup&gt;45&lt;/sup&gt;Ca dpm values were obtained. It is shown that the method presented gives reliable and consistent results. The recoveries of both isotopes are independent of the quench level in a large tSIE range, although five times more radioactivity is required for &lt;sup&gt;45&lt;/sup&gt;Ca than for &lt;sup&gt;57&lt;/sup&gt;Co to obtain accurate and reproducible results. The method has been used to study mechanisms of metal transport across biological interfaces.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2650
Fonte: | %3Ci%3EAnalytical+Biochemistry+224%3C%2Fi%3E%3A+92-99
Fonte: | info:eu-repo/semantics/restrictedAccess