Data/hora: 16/06/2024 06:49:55

Provedor de dados: | 27
País: | Belgium
Título: | Application of AFLP for taxonomic and epidemiological studies of &lt;i&gt;Photobacterium damselae&lt;/i&gt; subsp. &lt;i&gt;piscicida&lt;/i&gt;
Autores: | Thyssen, A.; Van Eygen, S.; Hauben, L.; Goris, J.; Swings, J.; Ollevier, F.P.
Data: | 2000
Ano: | 2000
Palavras-chave: | Epidemiology; Taxonomy.
Resumo: | A collection of 106 &lt;i&gt;Photobacterium damselae&lt;/i&gt; subsp. &lt;i&gt;piscicida&lt;/i&gt; strains and 19 &lt;i&gt;Photobacterium damselae&lt;/i&gt; subsp. &lt;i&gt;damselae&lt;/i&gt; strains, including reference and type strains, were genetically characterized using AFLP. The total genomic DNA of each bacterial strain was digested using restriction endonucleases &lt;i&gt;Hind&lt;/i&gt;III and &lt;i&gt;Taq&lt;/i&gt;I. Using numerical analysis, six clusters were recognized. The largest cluster (n=106) contained the majority of the strains tested and consisted exclusively of &lt;i&gt;Photobacterium damselae&lt;/i&gt; subsp. &lt;i&gt;piscicida&lt;/i&gt;. The &lt;i&gt;Photobacterium damselae&lt;/i&gt; subsp. &lt;i&gt;damselae&lt;/i&gt; strains fell outside this cluster. DNA-DNA hybridization experiments showed 77% DNA binding between the two subspecies, indicating a close genetic relationship. This clearly demonstrates the applicability of AFLP in studying the taxonomic position of &lt;i&gt;Photobacterium damselae&lt;/i&gt; subsp. &lt;i&gt;piscicida&lt;/i&gt;. In addition, AFLP proved to be a useful genotypic technique for epidemiological surveys of the pathogen, since it was able to discriminate between Mediterranean and Japanese &lt;i&gt;Photobacterium damselae&lt;/i&gt; subsp. &lt;i&gt;piscicida&lt;/i&gt; isolates.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.vliz.be/nl/open-marien-archief?module=ref&refid=9527
Fonte: | %3Ci%3EInt.+J.+Syst.+Bacteriol.+50%3C%2Fi%3E%3A+1013-1019
Fonte: | info:eu-repo/semantics/restrictedAccess