Data/hora: 01/06/2024 08:43:28

Provedor de dados: | 27
País: | Belgium
Título: | Measurement of the adenylate energy charge in &lt;i&gt;Nereis diversicolor&lt;/i&gt; and &lt;i&gt;Nephtys&lt;/i&gt; sp. (Polychaeta: Annelida): evaluation of the usefulness of AEC in pollution monitoring
Autores: | Verschraegen, K.; Herman, P.M.J.; Van Gansbeke, D.; Braeckman, A.
Data: | 1985
Ano: | 1985
Palavras-chave: | AMP ATP Efficiency Pollution monitoring Annelida [segmented worms] Nephtys Nereis diversicolor [estuary ragworm] Belgium; Zeeschelde.
Resumo: | ATP-content and adenylate energy charge (AEC) ratios were determined in two polychaete species (&lt;i&gt;Nereis diversicolor&lt;/i&gt; and &lt;i&gt;Nephtys&lt;/i&gt; sp.), sampled in ten stations along the heavily polluted Western Scheldt estuary (N. Belgium, S. Holland). The samples were taken between 27 December 1982 and 6 January 1983. &lt;i&gt;Nereis diversicolor&lt;/i&gt; was also sampled in an unpolluted brackish water pond, and subjected to artificial stress by drying the organisms on filter paper. Adenine nucleotide levels were determined using the firefly bioluminescence reaction. The light emission was measured with the integration mode. As the "Constant Light Signal" reagent used contained APM and probably ADP itself, both myokinase and pyruvatekinase had to be inactivated before the assay. The use of pepsin in acidic conditions to denaturate these enzymes is discussed. For both &lt;i&gt;N. diversicolor&lt;/i&gt; and &lt;i&gt;Nephtys&lt;/i&gt; sp., a significant relation between ATP-content and fresh weight is found in most samples. Within each species, ATP-content per g fresh weight is dependent on the sampling method, but is not significantly different between stations. It is higher in &lt;i&gt;Nephtys&lt;/i&gt; sp. from the W. Scheldt (130.8 10&lt;sup&gt;-5&lt;/sup&gt; g ATP/g fresh weight) than in &lt;i&gt;N. diversicolor&lt;/i&gt; from the Dievengat (83.9 10&lt;sup&gt;-5&lt;/sup&gt;g ATP/g fresh weight). This value in turn is significantly higher than that for &lt;i&gt;N. diversicolor&lt;/i&gt; in the W. Scheldt (64.0 10&lt;sup&gt;-5&lt;/sup&gt; g ATP/g fresh weight). In the drying experiment, AEC gradually decreases with increasing drying time. In the field, AEC is generally high and not significantly different between the three populations. AEC ratios also do not differ between stations within each population. It is argued that field monitoring of AEC in invertebrates is not valuable as a pollution assessment tool for two reasons. In polluted stations, only pollutant-resistant species will be found, and there is evidence that the maintenance of a stable population is impossible when the individuals constantly have low AECs.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3282
Relação: | info:eu-repo/semantics/altIdentifier/wos/A1985AJP8600003
Fonte: | %3Ci%3EMar.+Biol.+%28Berl.%29+86%3C%2Fi%3E%3A+233-240
Fonte: | info:eu-repo/semantics/restrictedAccess